Guttenbach M, Köhn F M, Engel W, Schmid M
Institut für Humangenetik, Universität Würzburg, Biozentrum, Am Hubland, 97074 Würzburg, Germany.
Biol Reprod. 2000 Dec;63(6):1727-9. doi: 10.1095/biolreprod63.6.1727.
In order to evaluate a possible paternal age effect, testicular sperm cells from three men aged 81, 82, and 83 yr were analyzed by two-color- and three-color-fluorescence in situ hybridization for disomy rates of chromosomes 1, 17, 18, X, and Y as well as for diploidy frequencies. A minimum of 1500 sperm cells per donor and probe was evaluated due to the low number of spermatozoa in the preparations. Diploidy and disomy frequencies were in the same range as found in men aged <30 yr, a slight increase only being noticed for XY nuclei.
为了评估可能存在的父亲年龄效应，对三名年龄分别为81岁、82岁和83岁男性的睾丸精子细胞进行了双色和三色荧光原位杂交分析，以检测1号、17号、18号染色体、X染色体和Y染色体的二体率以及二倍体频率。由于制备物中精子数量较少，每个供体和探针至少评估1500个精子细胞。二倍体和二体频率与年龄小于30岁男性的频率处于同一范围，仅XY核略有增加。
