男性年龄对不育人群精液质量、精子 DNA 碎片化和染色体异常的影响。
The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population.
机构信息
Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000 Sousse, Tunisia.
出版信息
J Assist Reprod Genet. 2011 May;28(5):425-32. doi: 10.1007/s10815-011-9537-5. Epub 2011 Feb 3.
Abstract
PURPOSE
To investigate the effects of male aging on semen quality, DNA fragmentation and chromosomal abnormalities in the spermatozoa of infertile patients and fertile men.
METHODS
Semen samples of 140 infertile patients (24-76 years) and 50 men with proven fertility (25-65 years) were analyzed according to WHO guidelines. DNA fragmentation was detected by TUNEL assay, while aneuploidy was assessed by FISH.
RESULTS
In the patient group, semen volume and vitality of spermatozoa decreased significantly with age, while sperm concentration showed a statistically significant increase with age. DNA fragmentation as well as disomy of sex chromosomes and disomy 8 did not show a statistically significant change with age. However, the diploidy rate was significantly increased with patient's age. In the control group, conventional semen parameters as well as DNA fragmentation and chromosomal abnormalities did not show a statistically significant with age.
CONCLUSION
Increased age in infertile men is associated with an increase in sperm concentration and diploidy, as well as a decline in semen volume and sperm vitality. However motility, morphology and DNA fragmentation are not affected by male age.
摘要
目的
研究男性年龄对不育患者和生育能力正常男性精子精液质量、DNA 碎片和染色体异常的影响。
方法
根据世界卫生组织（WHO）的指南，对 140 例不育患者（24-76 岁）和 50 例生育能力正常男性（25-65 岁）的精液样本进行分析。采用 TUNEL 法检测 DNA 碎片，用荧光原位杂交法（FISH）评估非整倍体。
结果
在患者组中，精子体积和活力随年龄增长显著下降，而精子浓度随年龄增长呈统计学意义上的显著增加。DNA 碎片以及性染色体和 8 号染色体三体均未随年龄发生统计学显著变化。然而，二倍体率随患者年龄的增长显著增加。在对照组中，常规精液参数以及 DNA 碎片和染色体异常均与年龄无统计学显著相关性。
结论
不育男性年龄的增加与精子浓度和二倍体率的增加以及精液量和精子活力的下降有关。然而，精子的运动能力、形态和 DNA 碎片不受男性年龄的影响。
相似文献
1
The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population.男性年龄对不育人群精液质量、精子 DNA 碎片化和染色体异常的影响。
J Assist Reprod Genet. 2011 May;28(5):425-32. doi: 10.1007/s10815-011-9537-5. Epub 2011 Feb 3.
2
Semen Quality is Associated with Sperm Aneuploidy and DNA Fragmentation in the United Arab Emirates Population.阿联酋人群的精液质量与精子非整倍体和 DNA 碎片化有关。
Genet Test Mol Biomarkers. 2020 Apr;24(4):195-203. doi: 10.1089/gtmb.2019.0180. Epub 2020 Mar 25.
3
Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men.精液中酶类抗氧化剂减少对突尼斯不育男性精子DNA片段化和精液质量的影响。
J Assist Reprod Genet. 2017 Mar;34(3):373-381. doi: 10.1007/s10815-013-9936-x. Epub 2013 Jan 25.
4
Correlation between semen parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men.荧光原位杂交技术研究不育男性精液参数与精子非整倍体率之间的相关性
Hum Reprod. 2000 Feb;15(2):351-65. doi: 10.1093/humrep/15.2.351.
5
Chromosomal aneuploidies and DNA fragmentation of human spermatozoa from patients exposed to perfluorinated compounds.接触全氟化合物患者的人类精子的染色体非整倍性和DNA片段化
Andrologia. 2015 Nov;47(9):1012-9. doi: 10.1111/and.12371. Epub 2014 Nov 8.
6
Increased numbers of DNA-damaged spermatozoa in samples presenting an elevated rate of numerical chromosome abnormalities.在存在染色体数目异常率升高的样本中，精子的 DNA 损伤数量增加。
Hum Reprod. 2013 Jun;28(6):1707-15. doi: 10.1093/humrep/det077. Epub 2013 Mar 22.
7
[Correlation of sperm DNA fragmentation index with age and semen parameters in infertile men].[不育男性精子DNA碎片化指数与年龄及精液参数的相关性]
Zhonghua Nan Ke Xue. 2018 Jul;24(7):608-612.
8
Aneuploidy and DNA fragmentation in morphologically abnormal sperm.形态异常精子中的非整倍体和DNA片段化
Int J Androl. 2010 Feb;33(1):e163-79. doi: 10.1111/j.1365-2605.2009.00982.x. Epub 2009 Sep 3.
9
Correlation study between sperm concentration, hyaluronic acid-binding capacity and sperm aneuploidy in Hungarian patients.匈牙利患者精子浓度、透明质酸结合能力与精子非整倍体的相关性研究。
Reprod Biomed Online. 2012 Dec;25(6):620-6. doi: 10.1016/j.rbmo.2012.08.003. Epub 2012 Sep 10.
10
Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos.不同类型精子染色体异常对胚胎植入前染色体构成的影响。
Fertil Steril. 2010 Sep;94(4):1380-1386. doi: 10.1016/j.fertnstert.2009.05.061. Epub 2009 Jul 15.
引用本文的文献
1
Increasing age in men is negatively associated with sperm quality and DNA integrity but not pregnancy outcomes in assisted reproductive technology.男性年龄增长与精子质量和DNA完整性呈负相关，但与辅助生殖技术中的妊娠结局无关。
Front Aging. 2025 May 21;6:1603916. doi: 10.3389/fragi.2025.1603916. eCollection 2025.
2
The Impact of Physical Exercise on Male Fertility Through Its Association with Various Processes and Aspects of Human Biology.体育锻炼通过与人类生物学的各种过程和方面的关联对男性生育能力的影响。
J Clin Med. 2025 May 14;14(10):3442. doi: 10.3390/jcm14103442.
3
A Review of Semen Analysis: Updates From the WHO Sixth Edition Manual and Advances in Male Fertility Assessment.精液分析综述：世界卫生组织第六版手册的更新及男性生育力评估进展
Cureus. 2024 Jun 29;16(6):e63485. doi: 10.7759/cureus.63485. eCollection 2024 Jun.
4
The effects of vitamin E supplementation on sperm parameters, chromatin integrity, and gene expression before and after freezing in aged mice.维生素E补充剂对老年小鼠冷冻前后精子参数、染色质完整性及基因表达的影响。
Clin Exp Reprod Med. 2024 Sep;51(3):213-224. doi: 10.5653/cerm.2023.06632. Epub 2024 Jun 10.
5
Paternal Age Amplifies Cryopreservation-Induced Stress in Human Spermatozoa.父龄加剧了人类精子冷冻保存诱导的应激。
Cells. 2024 Apr 4;13(7):625. doi: 10.3390/cells13070625.
6
Sperm chromatin structure assay (SCSA) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients.精子染色质结构分析（SCSA）和流式细胞术辅助的TUNEL分析，对大约10000名患者的大样本队列中精子DNA碎片化随年龄变化的情况提供了一致的评估。
Basic Clin Androl. 2023 Nov 30;33(1):33. doi: 10.1186/s12610-023-00208-9.
7
Reappraisal of the Concept of Accelerated Aging in Neurodegeneration and Beyond.神经退行性疾病及其他领域中加速衰老概念的再评估。
Cells. 2023 Oct 14;12(20):2451. doi: 10.3390/cells12202451.
8
The Effect of Age on Semen Quality Among Male Partners of Infertile Couples: An Observational Study in a Tertiary Care Center in Eastern India.年龄对不育夫妇男性伴侣精液质量的影响：印度东部一家三级护理中心的观察性研究。
Cureus. 2023 Aug 3;15(8):e42882. doi: 10.7759/cureus.42882. eCollection 2023 Aug.
9
The number of retrieved oocytes is correlated to sperm DNA fragmentation when predicting pregnancy outcomes.在预测妊娠结局时，回收的卵母细胞数量与精子DNA碎片率相关。
Am J Transl Res. 2023 May 15;15(5):3759-3765. eCollection 2023.
10
Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age.分析精子染色质包装和生殖生物标志物，以评估高龄男性的后果。
Front Endocrinol (Lausanne). 2023 Apr 14;14:1092603. doi: 10.3389/fendo.2023.1092603. eCollection 2023.
本文引用的文献
1
The effect of age on the expression of apoptosis biomarkers in human spermatozoa.年龄对人精子细胞中细胞凋亡生物标志物表达的影响。
Fertil Steril. 2010 Dec;94(7):2609-14. doi: 10.1016/j.fertnstert.2010.04.043. Epub 2010 Jun 12.
2
Sperm DNA damage: correlation to severity of semen abnormalities.精子DNA损伤：与精液异常严重程度的相关性。
Urology. 2009 Oct;74(4):789-93. doi: 10.1016/j.urology.2009.05.043. Epub 2009 Jul 30.
3
The correlation between male age, sperm quality and sperm DNA fragmentation in 320 men attending a fertility center.320名前往生育中心就诊男性的年龄、精子质量与精子DNA碎片化之间的相关性
J Assist Reprod Genet. 2009 Jan;26(1):41-6. doi: 10.1007/s10815-008-9277-3. Epub 2008 Nov 22.
4
Double probing of human spermatozoa for persistent histones, surplus cytoplasm, apoptosis and DNA fragmentation.对人类精子进行双探针检测，以检测持续存在的组蛋白、多余的细胞质、细胞凋亡和DNA片段化。
Reprod Biomed Online. 2008 Apr;16(4):570-9. doi: 10.1016/s1472-6483(10)60464-6.
5
The effects of male age on sperm DNA damage in an infertile population.
Reprod Biomed Online. 2007 Nov;15(5):514-9. doi: 10.1016/s1472-6483(10)60382-3.
6
The effects of age on DNA fragmentation, chromatin packaging and conventional semen parameters in spermatozoa of oligoasthenoteratozoospermic patients.年龄对少弱畸精子症患者精子中DNA片段化、染色质包装及常规精液参数的影响。
J Assist Reprod Genet. 2007 Oct;24(10):437-43. doi: 10.1007/s10815-007-9162-5. Epub 2007 Sep 4.
7
Frequency of human sperm carrying structural aberrations of chromosome 1 increases with advancing age.携带1号染色体结构畸变的人类精子的频率随年龄增长而增加。
Fertil Steril. 2007 May;87(5):1077-86. doi: 10.1016/j.fertnstert.2006.08.112. Epub 2007 Apr 11.
8
Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples.精子浓度正常男性的年龄与精液参数之间的关系：对6022份精液样本的分析
Andrologia. 2007 Apr;39(2):45-50. doi: 10.1111/j.1439-0272.2007.00761.x.
9
Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome.精子脱氧核糖核酸碎片作为辅助生殖技术结局的预后指标。
Fertil Steril. 2007 Jan;87(1):93-100. doi: 10.1016/j.fertnstert.2006.05.057. Epub 2006 Oct 30.
10
The effects of male age on sperm DNA damage in healthy non-smokers.健康非吸烟者中男性年龄对精子DNA损伤的影响。
Hum Reprod. 2007 Jan;22(1):180-7. doi: 10.1093/humrep/del338. Epub 2006 Oct 19.
Zotero 插件