Bigbee A J, Gosselink K L, Roy R R, Grindeland R E, Edgerton V R
Department of Neurobiology, University of California, Los Angeles 90095, California.
J Appl Physiol (1985). 2000 Dec;89(6):2174-8. doi: 10.1152/jappl.2000.89.6.2174.
Plasma growth hormone (GH) measured by immunoassay [immunoassayable GH (IGH)] and by tibial bioassay [bioassayable GH (BGH)] increases in humans in response to exercise. In rats, however, IGH does not change in response to exercise. The objective of this study was to determine the BGH response to an acute exercise bout in rats. The rats ran on a treadmill at a rate of 27 m/min for 15 min, after which plasma and pituitary hormones, including IGH and BGH, and plasma metabolites were measured. Plasma and pituitary IGH were unchanged from control groups after the acute exercise bout, whereas plasma BGH was increased by 300% and pituitary BGH was decreased by 50%. Plasma thyroxine and corticosterone levels were significantly increased after a single exercise bout, but plasma testosterone, 3,5, 3'-triiodothyronine, glucose, lactate, and triglyceride concentrations were unchanged. Given previous results from in situ nerve stimulation studies (Gosselink KL, Grindeland RE, Roy RR, Zhong H, Bigbee AJ, Grossman EJ, and Edgerton VR. J Appl Physiol 84: 1425-1430, 1998), these in vivo results are consistent with the rapid BGH response during exercise being induced by the activation of muscle afferents.
通过免疫测定法（可免疫测定的生长激素，IGH）和胫骨生物测定法（可生物测定的生长激素，BGH）测得的血浆生长激素（GH），在人类中会因运动而增加。然而，在大鼠中，IGH不会因运动而改变。本研究的目的是确定大鼠对急性运动发作的BGH反应。大鼠在跑步机上以27米/分钟的速度跑15分钟，之后测量血浆和垂体激素，包括IGH和BGH，以及血浆代谢物。急性运动发作后，血浆和垂体IGH与对照组相比没有变化，而血浆BGH增加了300%，垂体BGH减少了50%。单次运动发作后，血浆甲状腺素和皮质酮水平显著升高，但血浆睾酮、3,5,3'-三碘甲状腺原氨酸、葡萄糖、乳酸和甘油三酯浓度没有变化。鉴于之前原位神经刺激研究的结果（Gosselink KL、Grindeland RE、Roy RR、Zhong H、Bigbee AJ、Grossman EJ和Edgerton VR。《应用生理学杂志》84：1425 - 1430，1998），这些体内结果与运动期间快速的BGH反应是由肌肉传入神经的激活所诱导的一致。
