Zamani R, Semnanian S, Fathollahi Y, Hajizadeh S
Department of Physiology, School of Medical Sciences, Tarbiat Modarres University, P.O. Box 14115-111, Tehran, Iran.
Eur J Pharmacol. 2000 Nov 24;408(3):299-304. doi: 10.1016/s0014-2999(00)00794-9.
Laser-Doppler flowmetry was used to study cerebral cortical blood flow responses to morphine and naloxone in morphine-naive and -dependent rats. The experiments were performed in spontaneously breathing anesthetized rats. Morphine (10 mg/kg, i.p.) administration reduced regional cerebral blood flow in control, sham-operated and morphine-dependent rats, but the depressant effect of morphine in morphine-dependent animals was less than that in control and sham-operated groups. While naloxone (0.5 mg/kg, s.c.) had no considerable effect on regional cerebral blood flow in control and sham-operated groups, it increased regional blood flow in morphine dependent ones. The depressant effect of morphine in all groups and the enhancing effect of naloxone in morphine-dependent animals were not seen after local application of lidocaine at the recording site. This study may provide a framework to study the cellular and molecular mechanisms responsible for coupling neuronal electrical activity with regional alterations in blood flow during precipitation of morphine withdrawal.
采用激光多普勒血流仪研究了未用过吗啡和对吗啡成瘾的大鼠大脑皮质血流对吗啡和纳洛酮的反应。实验在自主呼吸的麻醉大鼠身上进行。腹腔注射吗啡（10毫克/千克）可降低对照组、假手术组和吗啡成瘾大鼠的局部脑血流量，但吗啡对吗啡成瘾动物的抑制作用小于对照组和假手术组。皮下注射纳洛酮（0.5毫克/千克）对对照组和假手术组的局部脑血流量没有显著影响，但可增加吗啡成瘾大鼠的局部脑血流量。在记录部位局部应用利多卡因后，吗啡在所有组中的抑制作用和纳洛酮在吗啡成瘾动物中的增强作用均未出现。本研究可能为研究在吗啡戒断过程中神经元电活动与血流局部改变耦合的细胞和分子机制提供一个框架。
