Adams R E, Wooten G F
Department of Neurology, University of Virginia School of Medicine, Charlottesville 22908.
Brain Res. 1990 Jun 4;518(1-2):6-10. doi: 10.1016/0006-8993(90)90946-9.
Regional cerebral glucose utilization (RCGU) and behavior during precipitated morphine withdrawal were studied in rats made dependent by either intracerebroventricular (i.c.v.) or subcutaneous (s.c.) administration of morphine. [14C]2-deoxy-D-glucose autoradiography revealed that RCGU increased in an anatomically related group of limbic and brainstem structures in rats that were in morphine withdrawal precipitated by naloxone administration compared to morphine-dependent controls that were not in precipitated withdrawal. Correlation of RCGU for 24 brain structures comparing i.c.v. vs s.c. morphine-treated rats was highly significant for groups in withdrawal and for controls (r values, 0.958 and 0.971, respectively). Withdrawal behaviors including autonomic signs of withdrawal, withdrawal jumping, and incidence of diarrhea were not different between the two groups in withdrawal (i.c.v. and s.c.). Weight loss during withdrawal increased (P less than 0.05) in rats made dependent by s.c. morphine administration compared to rats that received morphine by the i.c.v. route. Taken together, these results indicate that RCGU changes during morphine withdrawal result solely from effects of chronic morphine in the central nervous system, not in peripheral sites. The increased weight loss of s.c.-treated, morphine-dependent rats in withdrawal suggests an independent peripheral effect perhaps mediated by visceral opiate receptors.
通过脑室内（i.c.v.）或皮下（s.c.）注射吗啡使大鼠产生依赖性，研究了戒断诱发期吗啡戒断期间的局部脑葡萄糖利用（RCGU）和行为。[14C]2-脱氧-D-葡萄糖放射自显影显示，与未处于诱发戒断状态的吗啡依赖对照组相比，在注射纳洛酮诱发吗啡戒断的大鼠中，边缘系统和脑干结构的一组解剖学相关结构中的RCGU增加。比较经i.c.v.和s.c.吗啡处理的大鼠的24个脑结构的RCGU相关性，戒断组和对照组均高度显著（r值分别为0.958和0.971）。戒断行为包括戒断的自主体征、戒断跳跃和腹泻发生率，在两个戒断组（i.c.v.和s.c.）之间没有差异。与通过i.c.v.途径接受吗啡的大鼠相比，皮下注射吗啡产生依赖性的大鼠在戒断期间体重减轻增加（P小于0.05）。综上所述，这些结果表明，吗啡戒断期间的RCGU变化仅源于慢性吗啡对中枢神经系统的作用，而非外周部位。皮下注射吗啡依赖的大鼠在戒断期间体重减轻增加，提示可能由内脏阿片受体介导的独立外周效应。
