Bilotta J
Department of Psychology, Western Kentucky University, 1 Big Red Way, Bowling Green, KY 42101, USA.
Behav Brain Res. 2000 Nov 15;116(1):81-7. doi: 10.1016/s0166-4328(00)00264-3.
Studies across several species have demonstrated that the developing visual system is influenced by environmental conditions. This study examined the effects of abnormal lighting environments on zebrafish visual behavior. Zebrafish were raised under different lighting conditions from fertilization to 6 days postfertilization (dpf). These conditions included a normal light/dark cycle, constant dark, constant light, as well as exposure to various lighting cycles with intense light. Visual acuity was tested using the optomotor response to black and white vertical stripes of various widths. The results showed that visual acuity of the normal fish improved with age. In addition, fish raised in constant light had significantly lower visual acuity than fish raised under normal lighting conditions. Subjects raised in constant dark showed somewhat lower visual acuity than normal subjects when tested at 12-14 dpf, but the deficits were not as severe as those found in fish raised in constant light. Intense light rearing did not have any substantial effects on visual development, unless it was constant. In conclusion, under normal conditions, zebrafish visual acuity improves with age, supporting previous work on zebrafish development. In addition, zebrafish visual behavior is altered by abnormal lighting conditions; the most severe decrements occur when raised under constant light.
对多个物种的研究表明，发育中的视觉系统会受到环境条件的影响。本研究考察了异常光照环境对斑马鱼视觉行为的影响。斑马鱼从受精到受精后6天（dpf）在不同光照条件下饲养。这些条件包括正常的光/暗循环、持续黑暗、持续光照，以及暴露于各种强光光照循环下。使用对不同宽度黑白垂直条纹的视动反应来测试视力。结果表明，正常鱼类的视力随年龄增长而提高。此外，在持续光照下饲养的鱼的视力明显低于在正常光照条件下饲养的鱼。在持续黑暗中饲养的实验对象在12 - 14 dpf进行测试时，视力略低于正常实验对象，但缺陷程度不如在持续光照下饲养的鱼严重。除非光照持续，强光饲养对视觉发育没有任何实质性影响。总之，在正常条件下，斑马鱼的视力随年龄增长而提高，这支持了之前关于斑马鱼发育的研究。此外，斑马鱼的视觉行为会因异常光照条件而改变；在持续光照下饲养时视力下降最为严重。
