Krzykwa Julie C, Jeffries Marlo K
Texas Christian University, Department of Biology, 2800 S. University Drive, Fort Worth, TX 76129, United States.
MethodsX. 2020 Jun 20;7:100971. doi: 10.1016/j.mex.2020.100971. eCollection 2020.
The optomotor response (OMR) is a position stabilizing reflex to whole-field visual motion, demonstrated by fish instinctually moving to follow alternating black and white stripes. The OMR assay is used to assess the visual function of fish. While researchers have been using the OMR assay for years to evaluate the visual acuity of fish, no standardized methods exist. Therefore, the goal of the present study was to develop a standardized protocol for the measurement of OMR in larval fathead minnows, a common model species in ecotoxicology. Results indicate that OMR is a potential endpoint for the assessment of vision in larval fathead minnows and can be quantified as early as 9 dpf. When running the OMR assay, a 4-min acclimation period should be implemented. There did not appear to be a learning component to response latency; however, high variation in likely impeded the ability to detect significant differences.•Optomotor response of fathead minnow larvae can be quantified as early as 9 dpf.•When running the assay, a 4-min acclimation period should be implemented.•Due to high variation, response latency is not recommended for use as an endpoint.
视动反应（OMR）是一种针对全视野视觉运动的位置稳定反射，表现为鱼类本能地移动以跟随黑白交替的条纹。OMR测定法用于评估鱼类的视觉功能。尽管研究人员多年来一直使用OMR测定法来评估鱼类的视敏度，但目前尚无标准化方法。因此，本研究的目的是为幼体黑头呆鱼（一种生态毒理学中常用的模式物种）的OMR测量制定标准化方案。结果表明，OMR是评估幼体黑头呆鱼视觉的一个潜在终点，并且最早在9日龄时就可以进行量化。进行OMR测定时，应设置4分钟的适应期。反应潜伏期似乎不存在学习成分；然而，高变异性可能会妨碍检测显著差异的能力。
•黑头呆鱼幼体的视动反应最早在9日龄时就可以进行量化。
•进行测定时，应设置4分钟的适应期。
•由于变异性高，不建议将反应潜伏期用作一个终点。
