Nedić O, Nikolić J A, Hajduković-Dragojlović L, Todorović V, Masnikosa R
INEP - Institute for the Application of Nuclear Energy, Banatska 31b, 11080, Zemun, Yugoslavia.
Alcohol. 2000 Jul;21(3):223-9. doi: 10.1016/s0741-8329(00)00090-2.
The protein synthetic activity of the liver is diminished in cirrhosis. The aim of this study was to investigate possible changes in the serum IGF-IGFBP system among patients with alcoholic liver cirrhosis (ALC). The results obtained demonstrated that serum IGF-I and IGF-II concentrations were significantly lower in patients with ALC than in healthy persons (P=0.0008 for IGF-I and 0.0002 for IGF-II). The IGFBP profile was markedly altered and the 34 kDa IGFBP from patients had higher affinity towards 125I-IGF-II compared to the 34 kDa IGFBP of control individuals. Moreover, the 40-45 kDa IGFBP (in isolated complex with 125I-IGF-II) exhibited diminished interaction with concanavalin A, wheat germ, and breadfruit lectins. Modification of the glyco-component of the 40-45 kDa IGFBP seems to be an early event in ALC since change in reactivity towards lectins was noticed in patients with ALC classified as Child score A, whose serum IGF-I and IGF-II levels were within reference limits (the existence of carbohydrate microheterogeneity of this IGFBP was also assessed by lectin-affinity electrophoresis). It is possible that these biochemical alterations may affect the functional activity of the IGFs by changing the dynamics and distribution of these growth factors in the organism.
肝硬化时肝脏的蛋白质合成活性降低。本研究旨在调查酒精性肝硬化（ALC）患者血清胰岛素样生长因子-胰岛素样生长因子结合蛋白（IGF-IGFBP）系统可能存在的变化。所得结果表明，ALC患者的血清IGF-I和IGF-II浓度显著低于健康人（IGF-I为P = 0.0008，IGF-II为P = 0.0002）。IGFBP谱明显改变，与对照个体的34 kDa IGFBP相比，ALC患者的34 kDa IGFBP对125I-IGF-II具有更高的亲和力。此外，40 - 45 kDa IGFBP（与125I-IGF-II形成的分离复合物）与伴刀豆球蛋白A、麦胚凝集素和面包果凝集素的相互作用减弱。40 - 45 kDa IGFBP糖成分的修饰似乎是ALC中的一个早期事件，因为在Child评分A级的ALC患者中就注意到了对凝集素反应性的变化，这些患者的血清IGF-I和IGF-II水平在参考范围内（还通过凝集素亲和电泳评估了该IGFBP的碳水化合物微异质性的存在）。这些生化改变可能通过改变这些生长因子在机体中的动态和分布来影响IGF的功能活性。
