Kudo M, Watt R A, Moffat A C
Centre for Pharmaceutical Analysis, The School of Pharmacy, University of London, UK.
J Pharm Pharmacol. 2000 Oct;52(10):1271-7. doi: 10.1211/0022357001777252.
Glycosides from Digitalis are widely used for the treatment of various cardiac conditions. The potential for near-infrared (NIR) spectroscopy as a technique for the rapid identification of Digitalis purpurea was studied. If successful, this method would be advantageous over traditional methods which are destructive and time-consuming. It was possible to identify D. purpurea from other plants using a Maximum Distance in Wavelength Space statistical comparison method on standard normal variate-corrected, second-derivative spectra. Match values ranged from 1.65 to 2.26 for correct identification and were greater than 11.2 [corrected] for other plants. It was also possible to discriminate between different plant parts of D. purpurea, with match values ranging from 1.52 to 2-26 for leaves and greater than 29 for other parts of the same plant. The use of correlation coefficients and the Correlation in Wavelength Space methods proved less conclusive, with resulting values for leaves from different plants being very high, and in all but one case, above 0.9. A two-wavelength, nearest neighbours analysis was carried out for de-trended (baseline corrected), standard normal variate-corrected spectra at 1150 and 2160 nm. This resulted in the successful identification of unknown samples. NIR spectroscopy has the potential for the rapid identification of D. purpurea, and possibly for other natural products of pharmaceutical interest.
洋地黄中的糖苷被广泛用于治疗各种心脏疾病。研究了近红外（NIR）光谱技术用于快速鉴定毛地黄的潜力。如果成功，该方法将优于具有破坏性且耗时的传统方法。使用波长空间最大距离统计比较方法对标准正态变量校正的二阶导数光谱进行分析，有可能从其他植物中鉴别出毛地黄。正确鉴别的匹配值范围为1.65至2.26，而其他植物的匹配值大于11.2[校正后]。还能够区分毛地黄的不同植物部位，叶片的匹配值范围为1.52至2.26，同一植株其他部位的匹配值大于29。使用相关系数和波长空间相关性方法的结果不太确定，不同植物叶片的所得值非常高，除一个案例外，所有值均高于0.9。对1150和2160nm处经过去趋势（基线校正）、标准正态变量校正的光谱进行了双波长最近邻分析。这使得未知样品得以成功鉴定。近红外光谱技术有潜力快速鉴定毛地黄，也可能用于其他有药用价值的天然产物。
