Ikeda Y, Fujii Y, Umemura M, Hatakeyama T, Morita M, Yamazaki M
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
J Chromatogr A. 1996 Oct 11;746(2):255-60. doi: 10.1016/0021-9673(96)00340-8.
An analytical method for the determination of cardiac glycosides in Digitalis lanata leaves by reversed-phase thin-layer chromatography (RP-TLC) was developed. The procedure consisted of extraction of dry leaf powder with 50% methanol and clean-up by Sep-Pak cartridges prior to RP-TLC analysis. RP-TLC was performed on an octadecylsilyl bonded silica gel plate, using a developing solvent of acetonitrile-methanol-0.5 M NaCl (1:1:1) for primary glycosides and acetonitrile-methanol-0.5 M NaCl (12:7:9) for secondary glycosides. The plate was scanned with a reflectance densitometer at 225 nm. The quantitation was carried out by the internal standard method. The present method is reliable and relatively simple for the determination of cardiac glycosides in Digitalis lanata leaves.
建立了一种用反相薄层色谱法（RP-TLC）测定毛花洋地黄叶中强心苷的分析方法。该方法包括用50%甲醇提取干叶粉，并在RP-TLC分析前用Sep-Pak柱进行净化。RP-TLC在十八烷基硅烷键合硅胶板上进行，对于一级苷，使用乙腈-甲醇-0.5M氯化钠（1:1:1）的展开溶剂，对于二级苷，使用乙腈-甲醇-0.5M氯化钠（12:7:9）的展开溶剂。用反射密度计在225nm处扫描薄板。定量采用内标法进行。本方法用于测定毛花洋地黄叶中的强心苷可靠且相对简单。
