多种AraC/XylS家族激活剂介导的转录激活并不依赖于RNA聚合酶α亚基N端结构域中II类特异性激活决定簇。
Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit.
作者信息
Egan S M, Pease A J, Lang J, Li X, Rao V, Gillette W K, Ruiz R, Ramos J L, Wolf R E
机构信息
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA.
出版信息
J Bacteriol. 2000 Dec;182(24):7075-7. doi: 10.1128/JB.182.24.7075-7077.2000.
Abstract
The N-terminal domain of the RNA polymerase alpha subunit (alpha-NTD) was tested for a role in transcription activation by a variety of AraC/XylS family members. Based on substitutions at residues 162 to 165 and an extensive genetic screen we conclude that alpha-NTD is not an activation target for these activators.
摘要
通过多种AraC/XylS家族成员测试了RNA聚合酶α亚基的N端结构域（α-NTD）在转录激活中的作用。基于162至165位残基的替换以及广泛的遗传筛选，我们得出结论，α-NTD不是这些激活剂的激活靶点。
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