Amakura Y, Okada M, Tsuji S, Tonogai Y
National Institute of Health Sciences, Osaka Branch, Japan.
J Chromatogr A. 2000 Oct 27;896(1-2):87-93. doi: 10.1016/s0021-9673(00)00414-3.
A high-performance liquid chromatographic (HPLC) procedure based on an isocratic elution with photodiode array detection has been developed for a simple and rapid determination of ellagic acid (EA) in fresh and processed fruits. The homogenized sample was refluxed with methanol and then the extract was refined using a solid-phase cartridge before HPLC. We analyzed EA in 40 kinds of fresh fruits and 11 kinds of processed fruits by the developed method. EA was found in several berries, fueijoa, pineapple and pomegranate. This is the first occurrence of the detection of EA in bayberry, fueijoa and pineapple.
已开发出一种基于等度洗脱和光电二极管阵列检测的高效液相色谱（HPLC）方法，用于简单快速地测定新鲜水果和加工水果中的鞣花酸（EA）。将匀浆后的样品用甲醇回流，然后在进行HPLC分析之前，使用固相柱对提取物进行提纯。我们用所开发的方法分析了40种新鲜水果和11种加工水果中的EA。在几种浆果、费约果、菠萝和石榴中发现了EA。这是首次在杨梅、费约果和菠萝中检测到EA。
