Bertossi D, Kharouf S, d'Agostino A, Fior A, Bedogni A, Zancanaro C, Nocini P F
University of Verona, dental clinic, maxillofacial division, policlinico di Borgo Roma, Italie.
Ann Chir Plast Esthet. 2000 Oct;45(5):548-55; discussion 555-6.
At the end of the 1980's and at the beginning of the 1990's, free fat autografts were abandoned because of the poor long-term results. Two theories supported by hystological studies (the host replacement theory or the cell survival theory) have now been proposed to explain survival of fat tissue. They demonstrate that, when harvested by a correct technique, fat tissue presents the ideal characteristics to be grafted, providing a good soft-tissue substitute with a minimum follow-up of four years up to 6 years. The authors present a multiple stage lipofilling technique in 99 cases of facial rejuvenation by means of lipolayering. Injections are performed every 20 days and fat is stored fat at -30 degrees C with good aesthetic results and minimal morbidity with clinical follow-up at 4 and 6 years. Histological examination of fat stored for 20 days and for 8 months was also performed in 10 patients. Microscopic and ultrastructural examination demonstrated that stored adipocytes retain their anatomical features and, when injected in multiple stages, are adequate to obtain good clinical results. Our histological study combined with the clinical outcomes, suggests the advantage of several stage injection of stored fat (-30 degrees C).
在20世纪80年代末和90年代初，由于长期效果不佳，游离自体脂肪移植被摒弃。现在已经提出了两种得到组织学研究支持的理论（宿主替代理论或细胞存活理论）来解释脂肪组织的存活。这些理论表明，当采用正确的技术采集时，脂肪组织具有适合移植的理想特性，能提供良好的软组织替代物，至少随访四年至六年。作者介绍了一种在99例面部年轻化手术中通过脂肪分层进行多阶段脂肪填充的技术。每20天进行一次注射，脂肪储存在-30℃，取得了良好的美学效果，发病率极低，并进行了4年和6年的临床随访。还对10名患者储存20天和8个月的脂肪进行了组织学检查。显微镜和超微结构检查表明，储存的脂肪细胞保留了其解剖学特征，当分阶段注射时，足以获得良好的临床效果。我们的组织学研究结合临床结果，表明了分阶段注射储存脂肪（-30℃）的优势。
