Moussa B A
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
J Pharm Biomed Anal. 2000 Nov;23(6):1045-55. doi: 10.1016/s0731-7085(00)00384-8.
Simple, sensitive and accurate spectrophotometric methods for the determination of glafenine and metoclopramide hydrochloride are described. The first method is based on the oxidation of glafenine with iodic acid in strong acid medium to give a coloured diphenylbenzidine derivative and subsequent measurement of the coloured product at 509 nm. Beer's law is obeyed over the concentration range 2.5-20 microg ml(-1). The second method depends on the interaction of metoclopramide hydrochloride with p-dimethylaminocinnamaldehyde, to give a red coloured schiffs base with an absorbance maximum at 548 nm. Obedience to Beer's law is achieved over the concentration range 5-30 microg ml(-1). First-derivative method is used to overcome the slight interference of p-dimethylaminocinnamaldehyde reagent blank at the wavelength of measurement. Linearity between the peak heights at 576 nm versus concentration range 5-25 microg ml(-1) metoclopromide hydrochloride is obtained. The proposed methods have been successfully applied to the determination of these drugs in commercial products without interference. The validity of the methods is assessed by applying the standard addition technique, the relative standard deviation is less than 1%. The proposed methods are compared with reference methods with good agreement.
本文描述了测定格拉非宁和盐酸甲氧氯普胺的简单、灵敏且准确的分光光度法。第一种方法基于在强酸介质中用碘酸氧化格拉非宁，生成一种有色的二苯基联苯胺衍生物，随后在509nm处测量有色产物。在2.5 - 20μg ml⁻¹的浓度范围内符合比尔定律。第二种方法依赖于盐酸甲氧氯普胺与对二甲氨基肉桂醛的相互作用，生成一种红色的席夫碱，在548nm处有最大吸收。在5 - 30μg ml⁻¹的浓度范围内符合比尔定律。采用一阶导数法来克服在测量波长处对二甲氨基肉桂醛试剂空白的轻微干扰。在576nm处的峰高与5 - 25μg ml⁻¹盐酸甲氧氯普胺浓度范围之间获得线性关系。所提出的方法已成功应用于商业产品中这些药物的测定，无干扰。通过应用标准加入技术评估方法的有效性，相对标准偏差小于1%。将所提出的方法与参考方法进行比较，结果吻合良好。
