Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
J Fluoresc. 2011 Mar;21(2):739-45. doi: 10.1007/s10895-010-0764-4. Epub 2010 Nov 4.
A new, simple and accurate spectrofluorimetric method for the determination of metoclopramide hydrochloride was developed. The metoclopramide hydrochloride can remarkably enhance the luminescence intensity of the Tb(3+) ion doped in PMMA matrix at λ(ex)=360 nm in methanol at pH 6.9. The intensity of the emission band at 545 nm of Tb(3+) ion doped in PMMA matrix is increased due to the energy transfer from metoclopramide hydrochloride to Tb(3+) in the excited stated. The effect of different parameters, e.g., pH, temperature, Tb(3+) concentration, foreign ions that control the fluorescence intensity of the produced ion associate was critically investigated. The calibration curve of the emission intensity at 545 nm shows linear response of metoclopramide over a concentration range of 5 × 10(-5)-5.0 × 10(-8) M with detection limit of 8.7 × 10(-10) M. The method was used successfully for the determination of metoclopramide in pharmaceutical preparations and human serum. The average recovery of 99.48% with standard deviation of 0.32% and 96.98% with standard deviation of 0.4%, of pharmaceutical preparations and human serum respectively, were obtained which compared will with the results obtained from standard LC method of average recovery 99.04% and standard deviation of 0.6% and average recovery of 98.19% with standard deviation of 0.6% of pharmaceutical preparations and human serum, respectively.
开发了一种新的、简单且准确的用于测定盐酸甲氧氯普胺的分光荧光法。在甲醇中，pH 值为 6.9 时，盐酸甲氧氯普胺可显著增强掺杂在 PMMA 基质中的 Tb(3+)离子在 λ(ex)=360nm 处的发光强度。由于能量从激发态中的盐酸甲氧氯普胺转移到 Tb(3+)离子，掺杂在 PMMA 基质中的 Tb(3+)离子的发射带在 545nm 处的强度增强。研究了不同参数（例如 pH 值、温度、Tb(3+)浓度、控制产生离子缔合物荧光强度的外来离子）对荧光强度的影响。在 5×10(-5)-5.0×10(-8)M 的浓度范围内，发射强度在 545nm 处的校准曲线显示出线性响应，检测限为 8.7×10(-10)M。该方法成功用于药物制剂和人血清中盐酸甲氧氯普胺的测定。药物制剂和人血清的平均回收率分别为 99.48%，标准偏差为 0.32%和 96.98%，标准偏差为 0.4%，与标准 LC 方法得到的平均回收率 99.04%，标准偏差为 0.6%和平均回收率 98.19%，标准偏差为 0.6%相比。
