Cunha G R
Anat Rec. 1975 Jan;181(1):35-53. doi: 10.1002/ar.1091810104.
Fetal preputial glands from 14- to 17-day old mouse embryos undergo comparable growth, branching, and display similar degrees of cytodifferentiation when cultured in vitro on medium lacking hormones, on this medium in the presence of either of the fetal gondas, and on medium supplemented with testosterone dipropionate. Conversely, morphogenesis of these analgen is markedly suppressed by estrogen and an anti-androgen, Cyproterone acetate. These observations demonstrate that acquisition of androgen-sensitivity is a late event in the morphogenesis of the preputial gland and correlate with the developmental properties of preputial mesenchyme as demonstrated in earlier studies on the epithelio-mesenchymal interaction.
来自14至17日龄小鼠胚胎的胎儿包皮腺在缺乏激素的培养基上、在存在任何一种胎儿性腺的这种培养基上以及在补充有丙酸睾酮的培养基上进行体外培养时，会经历相当的生长、分支，并表现出相似程度的细胞分化。相反，这些原基的形态发生受到雌激素和一种抗雄激素——醋酸环丙孕酮的显著抑制。这些观察结果表明，雄激素敏感性的获得是包皮腺形态发生中的一个晚期事件，并且与包皮间充质的发育特性相关，正如早期关于上皮-间充质相互作用的研究所表明的那样。
