黏菌的线粒体RNA以非编码的3'聚（U）尾结尾。
Mitochondrial RNAs of myxomycetes terminate with non-encoded 3' poly(U) tails.
作者信息
Horton T L, Landweber L F
机构信息
Departments of Ecology and Evolutionary Biology, Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
出版信息
Nucleic Acids Res. 2000 Dec 1;28(23):4750-4. doi: 10.1093/nar/28.23.4750.
Abstract
We examined the 3' ends of edited RNAs from the myxomycetes Stemonitis flavogenita and Physarum polycephalum using a modified anchor PCR approach. Surprisingly, we found that poly(A) tails are missing from the cytochrome c oxidase subunit 1 mRNA (coI) from both species and the cytochrome c oxidase subunit 3 mRNA (cox3) from P. polycephalum. Instead, non-encoded poly(U) tails of varying length were discovered at the 3' ends of these transcripts. These are the first described examples of 3' poly(U) tails on mature mRNAs in any system.
摘要
我们使用改良的锚定PCR方法检测了黏菌黄柄绒泡菌和多头绒泡菌中编辑RNA的3'末端。令人惊讶的是，我们发现这两个物种的细胞色素c氧化酶亚基1 mRNA（coI）以及多头绒泡菌的细胞色素c氧化酶亚基3 mRNA（cox3）均缺少聚（A）尾。相反，在这些转录本的3'末端发现了长度各异的非编码聚（U）尾。这些是在任何系统中成熟mRNA上首次描述的3'聚（U）尾的例子。
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