多头绒泡菌线粒体基因组的完整DNA序列。

The complete DNA sequence of the mitochondrial genome of Physarum polycephalum.

作者信息

Takano H, Abe T, Sakurai R, Moriyama Y, Miyazawa Y, Nozaki H, Kawano S, Sasaki N, Kuroiwa T

机构信息

Department of Biological Science, Faculty of Science, Kumamoto University, Japan.

出版信息

Mol Gen Genet. 2001 Jan;264(5):539-45. doi: 10.1007/s004380000357.

DOI:10.1007/s004380000357

PMID:11212908

Abstract

The complete sequence of the mitochondrial DNA (mtDNA) of the true slime mold Physarun polycephalum has been determined. The mtDNA is a circular 62,862-bp molecule with an A+T content of 74.1%. A search with the program BLAST X identified the protein-coding regions. The mitochondrial genome of P. polycephalum was predicted to contain genes coding for 12 known proteins [for three cytochrome c oxidase subunits, apocytochrome b, two F1Fo-ATPase subunits, five NADH dehydrogenase (nad) subunits, and one ribosomal protein], two rRNA genes, and five tRNA genes. However, the predicted ORFs are not all in the same frame, because mitochondrial RNA in P. polycephalum undergoes RNA editing to produce functional RNAs. The nucleotide sequence of an nad7 cDNA showed that 51 nucleotides were inserted at 46 sites in the mRNA. No guide RNA-like sequences were observed in the mtDNA of P. polycephalum. Comparison with reported Physarum mtDNA sequences suggested that sites of RNA editing vary among strains. In the Physarum mtDNA, 20 ORFs of over 300 nucleotides were found and ORFs 14 19 are transcribed.

摘要

已确定多头绒泡菌这种黏菌的线粒体DNA（mtDNA）的完整序列。该mtDNA是一个环状的62,862碱基对分子，A+T含量为74.1%。用BLAST X程序进行搜索确定了蛋白质编码区。预测多头绒泡菌的线粒体基因组包含编码12种已知蛋白质的基因[三种细胞色素c氧化酶亚基、脱辅基细胞色素b、两种F1Fo - ATP合酶亚基、五种NADH脱氢酶（nad）亚基和一种核糖体蛋白]、两个rRNA基因和五个tRNA基因。然而，预测的开放阅读框并非都在同一读框内，因为多头绒泡菌中的线粒体RNA会经历RNA编辑以产生功能性RNA。一个nad7 cDNA的核苷酸序列显示，在mRNA的46个位点插入了51个核苷酸。在多头绒泡菌的mtDNA中未观察到类似引导RNA的序列。与已报道的绒泡菌mtDNA序列比较表明，RNA编辑位点在不同菌株间存在差异。在绒泡菌mtDNA中，发现了20个超过300个核苷酸的开放阅读框，且开放阅读框14至19被转录。