SNARE蛋白有助于突触传递的钙协同作用。
SNARE proteins contribute to calcium cooperativity of synaptic transmission.
作者信息
Stewart B A, Mohtashami M, Trimble W S, Boulianne G L
机构信息
Department of Molecular and Medical Genetics, Zoology, and Biochemistry, University of Toronto, 555 University Avenue, Toronto, ON, Canada.
出版信息
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13955-60. doi: 10.1073/pnas.250491397.
A hallmark of calcium-triggered synaptic transmission is the cooperative relationship between calcium and the amount of transmitter released. This relationship is thought to be important for improving the efficiency of synaptic vesicle exocytosis. Although it is generally held that cooperativity arises from the interaction of multiple calcium ions with a single calcium-sensing molecule, the precise molecular basis of this phenomenon is not known. The SNARE proteins are known to be critical for synaptic vesicle exocytosis. We therefore tested for a contribution of SNARE proteins to cooperativity by genetically reducing the levels of syntaxin IA and neuronal-synaptobrevin in Drosophila. Surprisingly, we found that reducing these SNARE proteins also reduced Ca(2+) cooperativity. Thus, SNARE proteins are important for determining the cooperative relationship between calcium and synaptic transmission.
钙触发的突触传递的一个标志是钙与释放的递质数量之间的协同关系。这种关系被认为对于提高突触小泡胞吐作用的效率很重要。尽管一般认为协同性源于多个钙离子与单个钙传感分子的相互作用，但这种现象的确切分子基础尚不清楚。已知SNARE蛋白对突触小泡胞吐作用至关重要。因此，我们通过基因手段降低果蝇中 syntaxin IA 和神经元突触小泡蛋白的水平，来测试SNARE蛋白对协同性的作用。令人惊讶的是，我们发现降低这些SNARE蛋白也会降低Ca(2+)协同性。因此SNARE蛋白对于确定钙与突触传递之间的协同关系很重要。
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