Sieliwanowicz B, Yamamoto M, Stasiuk L, Laskowski M
Biochemistry. 1975 Jan 14;14(1):39-46. doi: 10.1021/bi00672a007.
A limited digest of thymus DNA with salmon testes DNase was composed of fragments larger than tetranucleotides. With exhaustive digestion mono-, di-, and trinucleotides were formed. At the (3') omega terminus G predominated, its frequency ranged from approximately 35 to 72% and increased with decreasing size of fragments. The error caused by the ribose-containing contaminants of DNA is significant, and should not be neglected in the evaluation of nucleoside frequency at the terminal positions of fragments.
用鲑鱼精巢脱氧核糖核酸酶对胸腺脱氧核糖核酸进行有限消化，得到的片段大于四核苷酸。进行彻底消化时，会形成单核苷酸、二核苷酸和三核苷酸。在（3'）ω末端，鸟嘌呤占主导，其频率范围约为35%至72%，并随着片段大小的减小而增加。由含核糖的脱氧核糖核酸污染物引起的误差很大，在评估片段末端位置的核苷频率时不应忽视。
