Nakata K, Inoue Y, Harada J, Maeda N, Watanabe H, Tano Y, Shimomura Y, Harino S, Sawa M
Department of Ophthalmology, Osaka University Medical School, Osaka, Japan.
Ophthalmology. 2000 Dec;107(12):2167-71. doi: 10.1016/s0161-6420(00)00406-1.
To determine the frequency distribution of bacteria on the external surface of eyes of patients with atopic dermatitis (AD) and to investigate the relationship between the frequency of bacterial colonization and the grade of atopy or ocular diseases associated with AD.
Comparative cross-sectional study.
Thirty-six AD patients (mean age, 24.5 years) and 16 nonatopic, age-matched control participants (mean age, 25.5 years).
The eyelid margins and conjunctival sacs were scraped with sterile swabs. These samples were inoculated into aerobic and anaerobic culture media.
The frequency distribution of bacteria isolated from the eyelid margins and conjunctival sacs.
Bacteria isolated from AD patients were: Staphylococcus aureus in 21 of 36 patients (including methicillin-resistant Staphylococcus aureus in two patients); Staphylococcus epidermidis in two patients (including methicillin-resistant Staphylococcus epidermidis in one patient); other coagulase-negative Staphylococcus in six patients;alpha-streptococcus in three patients; Corynebacterium species in three patients; Neisseria species in two patients; and Propionibacterium acnes in one patient. From the nonatopic control participants, we isolated S. aureus in one patient, S. epidermidis in two patients and alpha-streptococcus in one patient. S. aureus was isolated from 67% of the AD patients, and any type of bacteria was isolated from 86% of the patients. These rates were significantly higher than those of nonatopic control participants (6% S. aureus and 25% any bacteria). There was no significant relationship between the frequency distribution of bacteria and the grade of atopy or associated ocular diseases.
High rates of bacterial colonization, especially S. aureus, were found in the conjunctival sacs and eyelid margins of AD patients. In case management of AD patients, this unique distribution of bacteria must be carefully considered.
确定特应性皮炎（AD）患者眼部外表面细菌的频率分布，并研究细菌定植频率与特应性等级或与AD相关的眼部疾病之间的关系。
比较性横断面研究。
36例AD患者（平均年龄24.5岁）和16例非特应性、年龄匹配的对照参与者（平均年龄25.5岁）。
用无菌拭子刮取眼睑边缘和结膜囊。将这些样本接种到需氧和厌氧培养基中。
从眼睑边缘和结膜囊中分离出的细菌的频率分布。
从AD患者中分离出的细菌有：36例患者中的21例为金黄色葡萄球菌（包括2例耐甲氧西林金黄色葡萄球菌）；2例患者为表皮葡萄球菌（包括1例耐甲氧西林表皮葡萄球菌）；6例患者为其他凝固酶阴性葡萄球菌；3例患者为α-链球菌；3例患者为棒状杆菌属；2例患者为奈瑟菌属；1例患者为痤疮丙酸杆菌。在非特应性对照参与者中，我们分离出1例金黄色葡萄球菌、2例表皮葡萄球菌和1例α-链球菌。67%的AD患者分离出金黄色葡萄球菌，86%的患者分离出任何类型的细菌。这些比率显著高于非特应性对照参与者（金黄色葡萄球菌6%，任何细菌25%）。细菌的频率分布与特应性等级或相关眼部疾病之间没有显著关系。
在AD患者的结膜囊和眼睑边缘发现了高比例细菌定植，尤其是金黄色葡萄球菌。在AD患者的病例管理中，必须仔细考虑这种独特的细菌分布情况。
