一种经过基因工程改造用于镍（II）生物累积的大肠杆菌菌株的构建与表征。
Construction and characterization of an Escherichia coli strain genetically engineered for Ni(II) bioaccumulation.
作者信息
Krishnaswamy R, Wilson D B
机构信息
Field of Environmental Toxicology, Cornell University, Ithaca, New York 14853, USA.
出版信息
Appl Environ Microbiol. 2000 Dec;66(12):5383-6. doi: 10.1128/AEM.66.12.5383-5386.2000.
Abstract
An Escherichia coli strain that accumulated Ni(II) was constructed by introducing the nixA gene (coding for a nickel transport system) from Helicobacter pylori into JM109 cells that expressed a glutathione S-transferase-pea metallothionein fusion protein. The resulting strain accumulated 15 micromol of Ni(II) per g (dry weight) from a 10 microM Ni(II) solution, four times the level taken up by JM109 cells. Ni(II) accumulation did not require an energy source, was inhibited by only 50% by 0.1 M NaCl, and occurred over the pH range from 3 to 9.
摘要
通过将幽门螺杆菌的nixA基因（编码镍转运系统）导入表达谷胱甘肽S-转移酶-豌豆金属硫蛋白融合蛋白的JM109细胞中，构建了一种积累镍（II）的大肠杆菌菌株。所得菌株从10微摩尔/升的镍（II）溶液中每克（干重）积累15微摩尔的镍（II），是JM109细胞吸收水平的四倍。镍（II）的积累不需要能量来源，仅被0.1摩尔/升的氯化钠抑制50%，并且在pH值为3至9的范围内发生。
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