Chen C Y, Lin T H
Department of Medical Technology, Foo-Yin Institute of Technology, and Graduate Institute of Medicine and School of Technology for Medical Sciences, Kaohsiung Medical College, Taiwan, Republic of China.
J Toxicol Environ Health A. 1998 May 8;54(1):37-47. doi: 10.1080/009841098159015.
It has been reported that nickel (Ni) crosses the human placenta and produces teratogenesis and embryotoxicity. In the present study, the effects of nickel on human term placentas were investigated. In time-course experiments, placental tissue was incubated for 3, 6, 12, or 24 h with 2.5 mM Ni. The viability as determined by glucose consumption rate did not show any significant change from 3 to 12 h, whereas the permeability, lipid peroxidation, and Ni concentration were significantly increased compared to the control. In concentration-response studies, placental explants were incubated with 0.5, 1.0, 2.5, or 5 mM Ni for 12 h. The viability did not change significantly, except for 5 mM Ni, but the permeability and lipid peroxidation increased markedly in a concentration-dependent manner. Treatment with ascorbic acid or Zn decreased placental lipid peroxidation and permeability induced by Ni, but had no effect on lowering the Ni tissue content. Data show that Ni is toxic as evidenced by lipid peroxidative damage to placental membrane, and this metabolic change may be responsible for decreased placental viability, altered permeability, and potential subsequent embryotoxicity.
据报道，镍（Ni）可穿过人类胎盘并产生致畸作用和胚胎毒性。在本研究中，对镍对足月人胎盘的影响进行了研究。在时间进程实验中，胎盘组织与2.5 mM镍一起孵育3、6、12或24小时。由葡萄糖消耗率测定的活力在3至12小时内未显示任何显著变化，而与对照组相比，通透性、脂质过氧化和镍浓度显著增加。在浓度-反应研究中，胎盘外植体与0.5、1.0、2.5或5 mM镍一起孵育12小时。除5 mM镍外，活力没有显著变化，但通透性和脂质过氧化以浓度依赖性方式显著增加。用抗坏血酸或锌处理可降低镍诱导的胎盘脂质过氧化和通透性，但对降低镍组织含量没有作用。数据表明，镍具有毒性，胎盘膜脂质过氧化损伤证明了这一点，这种代谢变化可能是胎盘活力降低、通透性改变以及潜在的后续胚胎毒性的原因。
