Fuse H, Takimura O, Murakami K, Yamaoka Y, Omori T
Chugoku National Industrial Research Institute, 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
Appl Environ Microbiol. 2000 Dec;66(12):5527-32. doi: 10.1128/AEM.66.12.5527-5532.2000.
Strain DMS-S1 isolated from seawater was able to utilize dimethyl sulfide (DMS) as a sulfur source only in the presence of light in a sulfur-lacking medium. Phylogenetic analysis based on 16S ribosomal DNA genes indicated that the strain was closely related to Marinobacterium georgiense. The strain produced dimethyl sulfoxide (DMSO), which was a main metabolite, and small amounts of formate and formaldehyde when grown on DMS as the sole sulfur source. The cells of the strain grown with succinate as a carbon source were able to use methyl mercaptan or methanesulfonate besides DMS but not DMSO or dimethyl sulfone as a sole sulfur source. DMS was transformed to DMSO primarily at wavelengths between 380 and 480 nm by heat-stable photosensitizers released by the strain. DMS was also degraded to formaldehyde in the presence of light by unidentified heat-stable factors released by the strain, and it appeared that strain DMS-S1 used the degradation products, which should be sulfite, sulfate, or methanesulfonate, as sulfur sources.
从海水中分离出的菌株DMS-S1仅在缺乏硫的培养基中且有光照的情况下，能够利用二甲基硫醚（DMS）作为硫源。基于16S核糖体DNA基因的系统发育分析表明，该菌株与乔治亚海杆菌密切相关。当以DMS作为唯一硫源生长时，该菌株产生二甲基亚砜（DMSO），这是一种主要代谢产物，以及少量甲酸盐和甲醛。以琥珀酸盐作为碳源生长的该菌株细胞，除了DMS外，还能够使用甲硫醇或甲磺酸盐作为硫源，但不能以DMSO或二甲基砜作为唯一硫源。DMS主要通过该菌株释放的热稳定光敏剂在380至480纳米波长之间转化为DMSO。在光照条件下，DMS也会被该菌株释放的未知热稳定因子降解为甲醛，并且似乎菌株DMS-S1利用降解产物（应为亚硫酸盐、硫酸盐或甲磺酸盐）作为硫源。
