González J M, Mayer F, Moran M A, Hodson R E, Whitman W B
Department of Microbiology, University of Georgia, Athens 30602, USA.
Int J Syst Bacteriol. 1997 Apr;47(2):369-76. doi: 10.1099/00207713-47-2-369.
Two numerically important bacteria in marine pulp mill effluent enrichment cultures were isolated. These organisms were gram-negative, rod-shaped, strictly aerobic bacteria. Isolate IRE-31T (T = type strain) produced hydrolytic enzymes for the breakdown of cellulose, xylan, chitin, gelatin, and Tween 80. It also utilized a variety of monosaccharides, disaccharides, amino acids, and volatile fatty acids for growth. Isolate KW-40T did not utilize natural polymers, but it could grow on a variety of monosaccharides, disaccharides, alcohols, and amino acids. It also utilized methanol and aromatic compounds. The surfaces of both organisms were covered by blebs and vesicles. 16S rRNA analyses placed both organisms in the gamma-3 subclass of the phylum Proteobacteria. They were related to Oceanospirillum linum, Marinomonas vaga, Pseudomonas putida, and Halomonas elongata, although a close association with any of these bacteria was not found. The guanine-plus-cytosine contents of strain IRE-31T and KW-40T were 57.6 and 54.9 mol%, respectively. On the basis of 16S rRNA sequence and phenotypic characterizations, these isolates were different enough so that they could be considered members of new genera. Thus, the following two new genera and species are proposed: Microbulbifer hydrolyticus, with type strain IRE-31 (= ATCC 700072), and Marinobacterium georgiense, with type strain KW-40 (= ATCC 700074).
从海洋纸浆厂废水富集培养物中分离出两种数量上重要的细菌。这些微生物是革兰氏阴性、杆状、严格需氧的细菌。分离株IRE-31T（T=模式菌株）产生用于分解纤维素、木聚糖、几丁质、明胶和吐温80的水解酶。它还利用多种单糖、双糖、氨基酸和挥发性脂肪酸进行生长。分离株KW-40T不利用天然聚合物，但它可以在多种单糖、双糖、醇类和氨基酸上生长。它还利用甲醇和芳香化合物。两种微生物的表面都覆盖有泡和囊泡。16S rRNA分析将这两种微生物置于变形菌门的γ-3亚类中。它们与亚麻海洋螺菌、漂泊海单胞菌、恶臭假单胞菌和嗜盐栖居单胞菌有关，尽管未发现与这些细菌中的任何一种有密切关联。菌株IRE-31T和KW-40T的鸟嘌呤加胞嘧啶含量分别为57.6和54.9摩尔%。基于16S rRNA序列和表型特征，这些分离株差异足够大，因此可以被认为是新属的成员。因此，提出了以下两个新属和新种：水解微小杆菌，模式菌株为IRE-31（=ATCC 700072），以及佐治亚海杆菌，模式菌株为KW-40（=ATCC 700074）。
