Prince M E, Nasser J G
Department of Otolaryngology-HNS, Dalhousie University, Halifax, Nova Scotia, Canada.
Comp Med. 2000 Oct;50(5):527-9.
Most animal models used to study the process of postnatal craniofacial growth require direct manipulation of the craniofacial area, a growth period, then evaluation of the area. However, the scar tissue associated with direct manipulation of the craniofacial structures can produce growth abnormalities that are unrelated to the manipulation itself. To avoid this confounding variable in the study of craniofacial growth, we developed an animal model that involves laryngotracheal separation in a young animal. Our procedure completely separates the trachea from the upper aerodigestive tract and removes the site of scar tissue formation from the region of investigation. The tracheal stomas of the goats we describe were maintained for as long as 9 months. Unlike human patients, goats with laryngotracheal separation require laryngectomy tubes to prevent life-threatening stenosis of the tracheal stoma. Here we describe the operative procedure and post-operative care required for this new animal model.
大多数用于研究出生后颅面生长过程的动物模型需要直接对颅面区域进行操作，经过一个生长阶段，然后对该区域进行评估。然而，与直接操作颅面结构相关的瘢痕组织会产生与操作本身无关的生长异常。为了在颅面生长研究中避免这种混杂变量，我们开发了一种在幼小动物中进行喉气管分离的动物模型。我们的手术将气管与上呼吸道消化道完全分离，并从研究区域移除瘢痕组织形成部位。我们所描述的山羊气管造口维持了长达9个月。与人类患者不同，喉气管分离的山羊需要喉切除管来防止危及生命的气管造口狭窄。在此我们描述这种新动物模型所需的手术操作和术后护理。
