Marañón C, Planelles L, Alonso C, López M C
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina 'López Neyra', CSIC, Calle Ventanilla 11, 18001 Granada, Spain.
Int Immunol. 2000 Dec;12(12):1685-93. doi: 10.1093/intimm/12.12.1685.
The Trypanosoma cruzi HSP70 recombinant protein has the capacity to stimulate splenocytes or lymph node cells from naive mice in a non-haplotype-restricted way. The proliferative response is abolished by proteinase K digestion and by specific anti-HSP70 antibodies. The induced stimulation index was maximal after 24 h of incubation with the protein. This stimulation leads to cell death in a Fas-Fas ligand-independent way. The phenotype of the expanded cells was CD3(+) TCRalphass(+) CD4(+). HSP70-responsive cells express a broad range of cytokines including IFN-gamma, IL-2 and tumor necrosis factor-alpha. After 48 h of incubation with HSP70 there was a significant increase in relative intracellular levels of CD3 TCRalphass receptors. The expanded CD4(+) cell population expressed CD25; however, in contrast to concanavalin A-treated culture, delayed CD44 expression was observed.
克氏锥虫热休克蛋白70重组蛋白能够以非单倍型限制的方式刺激未接触过抗原的小鼠的脾细胞或淋巴结细胞。蛋白酶K消化和特异性抗热休克蛋白70抗体可消除增殖反应。与该蛋白孵育24小时后，诱导的刺激指数最大。这种刺激以不依赖Fas-Fas配体的方式导致细胞死亡。扩增细胞的表型为CD3(+) TCRαβ(+) CD4(+)。对热休克蛋白70有反应的细胞表达多种细胞因子，包括γ干扰素、白细胞介素-2和肿瘤坏死因子-α。与热休克蛋白70孵育48小时后，CD3 TCRαβ受体的相对细胞内水平显著增加。扩增的CD4(+)细胞群体表达CD25；然而，与伴刀豆球蛋白A处理的培养物相比，观察到CD44表达延迟。
