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用与KMP11蛋白融合的克氏锥虫热休克蛋白70进行DNA免疫可引发针对该抗原的细胞毒性和体液免疫反应，并带来保护作用。

DNA immunization with Trypanosoma cruzi HSP70 fused to the KMP11 protein elicits a cytotoxic and humoral immune response against the antigen and leads to protection.

作者信息

Planelles L, Thomas M C, Alonso C, López M C

机构信息

Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra, CSIC, 18001 Granada, Spain.

出版信息

Infect Immun. 2001 Oct;69(10):6558-63. doi: 10.1128/IAI.69.10.6558-6563.2001.

DOI:10.1128/IAI.69.10.6558-6563.2001
PMID:11553607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC98798/
Abstract

Murine immunization with Trypanosoma cruzi KMP11-HSP70 fused genes but not the KMP11 gene alone elicited both an immunoglobulin G2a long-lasting humoral immune response against KMP11 protein and activation of CD8+ cytotoxic T lymphocytes specific for two KMP11 peptides containing A2 motifs. Moreover, protection against the parasite challenge was observed after immunization with the chimeric gene.

摘要

用克氏锥虫KMP11 - HSP70融合基因而非单独的KMP11基因对小鼠进行免疫，可引发针对KMP11蛋白的免疫球蛋白G2a持久体液免疫反应，以及针对两种含A2基序的KMP11肽的CD8 + 细胞毒性T淋巴细胞的激活。此外，用嵌合基因免疫后观察到对寄生虫攻击的保护作用。

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1
DNA immunization with Trypanosoma cruzi HSP70 fused to the KMP11 protein elicits a cytotoxic and humoral immune response against the antigen and leads to protection.用与KMP11蛋白融合的克氏锥虫热休克蛋白70进行DNA免疫可引发针对该抗原的细胞毒性和体液免疫反应，并带来保护作用。
Infect Immun. 2001 Oct;69(10):6558-63. doi: 10.1128/IAI.69.10.6558-6563.2001.
2
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Mapping of the antigenic determinants of the T. cruzi kinetoplastid membrane protein-11. Identification of a linear epitope specifically recognized by human Chagasic sera.克氏锥虫动质体膜蛋白-11抗原决定簇的定位。鉴定人类恰加斯病血清特异性识别的线性表位。
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Molecular characterization of KMP11 from Trypanosoma cruzi: a cytoskeleton-associated protein regulated at the translational level.克氏锥虫KMP11的分子特征：一种在翻译水平受调控的细胞骨架相关蛋白。
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In vivo cytotoxic T lymphocyte elicitation by mycobacterial heat shock protein 70 fusion proteins maps to a discrete domain and is CD4(+) T cell independent.分枝杆菌热休克蛋白70融合蛋白在体内引发细胞毒性T淋巴细胞定位于一个离散结构域，且不依赖CD4(+) T细胞。
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Vaccination with trypomastigote surface antigen 1-encoding plasmid DNA confers protection against lethal Trypanosoma cruzi infection.用编码锥鞭毛体表面抗原1的质粒DNA进行疫苗接种可提供针对致命性克氏锥虫感染的保护作用。
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Cross-priming of CTL responses in vivo does not require antigenic peptides in the endoplasmic reticulum of immunizing cells.体内细胞毒性T淋巴细胞（CTL）反应的交叉启动并不需要免疫细胞内质网中的抗原肽。
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Humoral and cellular immune responses to synthetic peptides of the Leishmania donovani kinetoplastid membrane protein-11.针对杜氏利什曼原虫动质体膜蛋白11合成肽的体液免疫和细胞免疫反应
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Development of Th1 and Th2 populations and the nature of immune responses to hepatitis B virus DNA vaccines can be modulated by codelivery of various cytokine genes.通过共递送各种细胞因子基因，可以调节Th1和Th2细胞群的发育以及对乙型肝炎病毒DNA疫苗的免疫反应性质。
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