Wang Q, Khillan J, Gadue P, Nishikura K
Wistar Institute, Philadelphia, PA 19104, USA.
Science. 2000 Dec 1;290(5497):1765-8. doi: 10.1126/science.290.5497.1765.
The members of the ADAR (adenosine deaminase acting on RNA) gene family are involved in site-selective RNA editing that changes adenosine residues of target substrate RNAs to inosine. Analysis of staged chimeric mouse embryos with a high contribution from embryonic stem cells with a functional null allele for ADAR1 revealed a heterozygous embryonic-lethal phenotype. Most ADAR1+/- chimeric embryos died before embryonic day 14 with defects in the hematopoietic system. Our results suggest the importance of regulated levels of ADAR1 expression, which is critical for embryonic erythropoiesis in the liver.
ADAR（作用于RNA的腺苷脱氨酶）基因家族的成员参与位点选择性RNA编辑，该编辑可将靶底物RNA的腺苷残基转变为肌苷。对具有ADAR1功能无效等位基因的胚胎干细胞贡献度高的分期嵌合小鼠胚胎进行分析，发现了杂合子胚胎致死表型。大多数ADAR1+/-嵌合胚胎在胚胎第14天之前死亡，造血系统存在缺陷。我们的结果表明，ADAR1表达水平受到调控具有重要意义，这对肝脏中的胚胎红细胞生成至关重要。
