Brazelton T R, Rossi F M, Keshet G I, Blau H M
Department of Molecular Pharmacology, CCSR 4215, 269 Campus Drive, Stanford University, Stanford, CA 94305-5175, USA.
Science. 2000 Dec 1;290(5497):1775-9. doi: 10.1126/science.290.5497.1775.
After intravascular delivery of genetically marked adult mouse bone marrow into lethally irradiated normal adult hosts, donor-derived cells expressing neuronal proteins (neuronal phenotypes) developed in the central nervous system. Flow cytometry revealed a population of donor-derived cells in the brain with characteristics distinct from bone marrow. Confocal microscopy of individual cells showed that hundreds of marrow-derived cells in brain sections expressed gene products typical of neurons (NeuN, 200-kilodalton neurofilament, and class III beta-tubulin) and were able to activate the transcription factor cAMP response element-binding protein (CREB). The generation of neuronal phenotypes in the adult brain 1 to 6 months after an adult bone marrow transplant demonstrates a remarkable plasticity of adult tissues with potential clinical applications.
将经基因标记的成年小鼠骨髓通过血管内注射到接受致死剂量照射的正常成年宿主后，中枢神经系统中出现了表达神经元蛋白（神经元表型）的供体来源细胞。流式细胞术显示，大脑中存在一群具有不同于骨髓特征的供体来源细胞。对单个细胞进行共聚焦显微镜检查发现，脑切片中有数百个骨髓来源的细胞表达典型的神经元基因产物（神经元核抗原、200千道尔顿神经丝和III类β-微管蛋白），并且能够激活转录因子环磷酸腺苷反应元件结合蛋白（CREB）。成年骨髓移植后1至6个月在成体大脑中产生神经元表型，这证明了成体组织具有显著的可塑性，具有潜在的临床应用价值。
