碱性成纤维细胞生长因子刺激前后，软骨发育不全和正常小鼠软骨细胞的体外增殖情况。
In vitro proliferation of achondroplastic and normal mouse chondrocytes, before and after basic fibroblast growth factor stimulation.
作者信息
Argentin G, Cicchetti R
机构信息
Department of Public Health and Cellular Biology, University of Rome Tor Vergata, Italy.
出版信息
Cell Prolif. 2000 Dec;33(6):397-405. doi: 10.1046/j.1365-2184.2000.00185.x.
Achondroplasia in mice is a recessive genetic disorder, characterized by disproportionate dwarfism with reduced bone growth. The cause of this chondrodystrophy is unknown. In this study normal and achondroplastic mouse chondrocytes were cultured in monolayer primary culture, their differentiation was verified by immunofluorescence and their growth was compared. The results showed that achondroplastic cells exhibited a higher proliferative activity than control cells of the same age, confirmed also by a thymidine incorporation assay. Furthermore, basic fibroblast growth factor treatment was found to induce a strong increase in growth of normal mouse chondrocytes, while it did not stimulate statistically significant proliferation of achondroplastic mouse cells. We suppose that this different growth rate could play a role in achondroplastic phenotype development.
小鼠软骨发育不全是一种隐性遗传疾病，其特征为不成比例的侏儒症以及骨骼生长减缓。这种软骨发育不良的病因尚不清楚。在本研究中，正常和软骨发育不全小鼠的软骨细胞进行了单层原代培养，通过免疫荧光验证其分化情况，并比较了它们的生长情况。结果显示，软骨发育不全细胞比同年龄的对照细胞表现出更高的增殖活性，这也通过胸腺嘧啶核苷掺入试验得到证实。此外，发现碱性成纤维细胞生长因子处理可诱导正常小鼠软骨细胞的生长显著增加，而对软骨发育不全小鼠细胞的增殖没有统计学上的显著刺激作用。我们推测这种不同的生长速率可能在软骨发育不全表型的发展中起作用。
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