Kang C J, Lee M G, Cho Y S, Lee J W, Kyung Y J, Shin J S, Kim E S, Kim J K
Department of Biology, Korea University, Seoul.
Mol Cells. 2000 Oct 31;10(5):579-83. doi: 10.1007/s10059-000-0579-2.
A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5' untranslated region (UTR) and 139 bp of 3' UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.
分离得到香叶天竺葵（Pelargonium graveolens）叶绿体翻译延伸因子EF-Tu（tufA）的cDNA。香叶天竺葵tufA cDNA长1584 bp，5'非翻译区（UTR）有20 bp，3'UTR有139 bp。它编码474个氨基酸，包括一个65个氨基酸的假定叶绿体转运肽。香叶天竺葵tufA cDNA推导的多肽在其N端区域含有四个GTP结合序列，在C端区域含有两个叶绿体EF-Tu特征区域。成熟香叶天竺葵叶绿体EF-Tu蛋白的预测分子量约为45000，其与烟草、豌豆、拟南芥、水稻和大豆的叶绿体EF-Tu蛋白的氨基酸序列同一性在85%至91%之间。香叶天竺葵tufA似乎像拟南芥和水稻一样以单拷贝基因形式存在，而其他已知双子叶植物在其核基因组中有多个拷贝。
