Murayama Y, Matsubayashi T, Sugita M, Sugiura M
Center for Gene Research, Nagoya University, Japan.
Plant Mol Biol. 1993 Aug;22(5):767-74. doi: 10.1007/BF00027363.
We have purified a chloroplast elongation factor Tu (EF-Tu) from tobacco (Nicotiana tabacum) and determined its N-terminal amino acid sequence. Two distinct cDNAs encoding EF-Tu were isolated from a leaf cDNA library of N. sylvestris (the female progenitor of N. tabacum) using an oligonucleotide probe based on the EF-Tu protein sequence. The cDNA sequence and genomic Southern analyses revealed that tobacco chloroplast EF-Tu is encoded by two distinct genes in the nuclear genome of N. sylvestris. We designated the corresponding gene products EF-Tu A and B. The mature polypeptides of EF-Tu A and B are 408 amino acids long and share 95.3% amino acid identity. They show 75-78% amino acid identity with cyanobacterial and chloroplast-encoded EF-Tu species.
我们从烟草（Nicotiana tabacum）中纯化了一种叶绿体延伸因子Tu（EF-Tu），并测定了其N端氨基酸序列。利用基于EF-Tu蛋白序列的寡核苷酸探针，从林烟草（烟草的雌性祖先）的叶片cDNA文库中分离出两个编码EF-Tu的不同cDNA。cDNA序列和基因组Southern分析表明，烟草叶绿体EF-Tu由林烟草核基因组中的两个不同基因编码。我们将相应的基因产物命名为EF-Tu A和B。EF-Tu A和B的成熟多肽长度为408个氨基酸，氨基酸同一性为95.3%。它们与蓝细菌和叶绿体编码的EF-Tu物种的氨基酸同一性为75-78%。
