Van Den Heuvel C, Finnie J W, Blumbergs P C, Manavis J, Jones N R, Reilly P L, Pereira R A
Department of Pathology, University of Adelaide, South Australia, Australia.
J Neurotrauma. 2000 Nov;17(11):1041-53. doi: 10.1089/neu.2000.17.1041.
The aim of this study was to assess and quantitate topographically the effects of posttraumatic intravenous magnesium sulphate (MgSO4) on neuronal perikaryal APP antigen and messenger RNA (mRNA) expression in sheep brains 2 h after a controlled focal head impact. The percentage brain area with APP immunoreactive neuronal perikarya was 71, 56, 27.5 and 5.5%, respectively, in MgSO4-treated head-injured animals, head-injured animals without any treatment, MgSO4 treated nonimpacted animals, and nontreated nonimpacted control sheep. Although there was no statistically significant difference in APP immunoreactive neuronal perikarya in the MgSO4-treated HI group (mean 71%) compared to the HI group without any treatment (mean 56%), northern analysis showed that there was a 2.3-+/-0.2-fold increase in APP mRNA in the thalamus of treated impacted animals compared to untreated impacted animals (p < 0.005). However, MgSO4 treated nonimpacted control animals also showed a 1.6-+/-0.1-fold increase in APP mRNA compared to untreated nonimpacted controls (p < 0.005). MgSO4 therapy results in upregulation of neuronal APP mRNA and APP expression that is quantitatively greater following a focal head impact.
本研究的目的是在可控性局灶性头部撞击后2小时，对创伤后静脉注射硫酸镁（MgSO4）对绵羊脑神经元胞体APP抗原和信使核糖核酸（mRNA）表达的影响进行地形学评估和定量分析。在接受MgSO4治疗的头部受伤动物、未接受任何治疗的头部受伤动物、接受MgSO4治疗的未受撞击动物以及未治疗的未受撞击对照绵羊中，APP免疫反应性神经元胞体所在的脑区百分比分别为71%、56%、27.5%和5.5%。尽管与未接受任何治疗的头部受伤组（平均56%）相比，接受MgSO4治疗的头部受伤组（平均71%）中APP免疫反应性神经元胞体并无统计学上的显著差异，但Northern分析显示，与未治疗的受撞击动物相比，接受治疗的受撞击动物丘脑内的APP mRNA增加了2.3±0.2倍（p<0.005）。然而，与未治疗的未受撞击对照相比，接受MgSO4治疗的未受撞击对照动物的APP mRNA也增加了1.6±0.1倍（p<0.005）。MgSO4治疗导致神经元APP mRNA和APP表达上调，在局灶性头部撞击后上调程度在数量上更大。
