Blumbergs P, Finnie J, Manavis J, Lewis S, Jones N, Reilly P, Pereira R
Department of Pathology, University of Adelaide, Institute of Medical and Veterinary Science, South Australia.
J Clin Neurosci. 2000 Mar;7(2):140-5. doi: 10.1054/jocn.1999.0168.
Amyloid precursor protein (APP), a membrane spanning glycoprotein which plays an important role in neuronal growth and synaptic plasticity, is increased after traumatic brain injury (TBI) and has been used as a sensitive marker of neuronal damage in an adult sheep head impact model. We hypothesised that APP expression would similarly be increased in lambs, suggesting that in the immature injured brain APP is also upregulated as an acute phase response to trauma. Ten anaesthetised and ventilated 4-5 week old Merino lambs sustained a left temporal head impact from a humane stunner. At 2 h after impact, there was widespread and intense neuronal cell body APP immunoreactivity which was more widely distributed than axonal APP. APP messenger RNA (mRNA) expression was also markedly increased with a distribution similar to that of APP antigen. These results demonstrate that APP antigen and mRNA are sensitive early indicators of TBI in paediatric cases.
淀粉样前体蛋白（APP）是一种跨膜糖蛋白，在神经元生长和突触可塑性中起重要作用。创伤性脑损伤（TBI）后其水平会升高，并已在成年绵羊头部撞击模型中用作神经元损伤的敏感标志物。我们推测，羔羊中APP的表达也会同样升高，这表明在未成熟的受伤大脑中，APP也作为对创伤的急性期反应而上调。十只4-5周龄经麻醉和通气的美利奴羔羊受到人道击晕器造成的左颞部头部撞击。撞击后2小时，存在广泛且强烈的神经元细胞体APP免疫反应性，其分布比轴突APP更广泛。APP信使核糖核酸（mRNA）表达也显著增加，其分布与APP抗原相似。这些结果表明，APP抗原和mRNA是儿科TBI的敏感早期指标。
