Bogaert D, Syrogiannopoulos G A, Grivea I N, de Groot R, Beratis N G, Hermans P W
Department of Pediatrics, Sophia Children's Hospital, Erasmus University Rotterdam, Rotterdam, The Netherlands.
J Clin Microbiol. 2000 Dec;38(12):4361-6. doi: 10.1128/JCM.38.12.4361-4366.2000.
A total of 145 penicillin-nonsusceptible Streptococcus pneumoniae strains were isolated from young carriers in Greece and analyzed by antibiotic susceptibility testing, serotyping, restriction fragment end labeling (RFEL), and penicillin-binding protein (PBP) genotyping. The serotypes 23A and 23F (54%), 19A and 19F (25%), 9V (5%), 15A, 15B, and 15C (4%), 6A and 6B (4%), and 21 (4%) were most prevalent in this collection. Fifty-three distinct RFEL types were identified. Sixteen different RFEL clusters, harboring 2 to 32 strains each, accounted for 82% of all strains. Eight of these genetic clusters representing 60% of the strains were previously identified in other countries. A predominant lineage of 66 strains (46%) harboring five RFEL types and the serotypes 19F and 23F was closely related to the pandemic clone Spain(23F)-1 (genetic relatedness of > or =85%). Another lineage, representing 11 strains, showed close genetic relatedness to the pandemic clone France(9V)-3. Another lineage of 8 serotype 21 strains was Greece specific since the RFEL types were not observed in an international collection of 193 genotypes from 16 different countries. Characterization of the PBP genes pbp1a, pbp2b, and pbp2x revealed 20 distinct PBP genotypes of which PBP type 1-1-1, initially observed in the pandemic clones 23F and 9V, was predominantly present in 11 RFEL types in this Greek collection of penicillin-nonsusceptible strains (55%). Sixteen PBP types covering 52 strains (36%) were Greece specific. This study underlines the strong contribution of penicillin-resistant international clones to the prevalence and spread of penicillin-nonsusceptible pneumococci among young children in Greece.
从希腊的年轻携带者中总共分离出145株对青霉素不敏感的肺炎链球菌菌株，并通过抗生素敏感性测试、血清分型、限制性片段末端标记（RFEL）和青霉素结合蛋白（PBP）基因分型进行分析。血清型23A和23F（54%）、19A和19F（25%）、9V（5%）、15A、15B和15C（4%）、6A和6B（4%）以及21（4%）在该样本中最为常见。鉴定出53种不同的RFEL类型。16个不同的RFEL簇，每个簇包含2至32个菌株，占所有菌株的82%。这些遗传簇中有8个代表60%的菌株，此前在其他国家已被鉴定。一个由66个菌株（46%）组成的主要谱系，包含5种RFEL类型以及血清型19F和23F，与大流行克隆西班牙（23F）-1密切相关（遗传相关性≥85%）。另一个由11个菌株组成的谱系与大流行克隆法国（9V）-3显示出密切的遗传相关性。另一个由8个血清型21菌株组成的谱系是希腊特有的，因为在来自16个不同国家的193种基因型的国际样本中未观察到这些RFEL类型。对PBP基因pbp1a、pbp2b和pbp2x的表征揭示了20种不同的PBP基因型，其中最初在大流行克隆23F和9V中观察到的PBP 1-1-1型在这个希腊对青霉素不敏感菌株样本的11种RFEL类型中占主导地位（55%）。16种PBP类型涵盖52个菌株（36%）是希腊特有的。这项研究强调了耐青霉素国际克隆对希腊幼儿中对青霉素不敏感肺炎球菌的流行和传播的重要贡献。
