Effenberger F, Förster S, Wajant H
Institut für Organische Chemie der Universität Stuttgart, Pfaffenwaldring 55, D-70569 Stuttgart, Germany.
Curr Opin Biotechnol. 2000 Dec;11(6):532-9. doi: 10.1016/s0958-1669(00)00141-5.
(R)- as well as (S)-cyanohydrins are now easily available as a result of the excellent accessibility, the relatively high stability and the easy handling of hydroxynitrile lyases (HNLs). The optimization of reaction conditions (solvent, temperature, and using site-directed mutagenesis, etc.) has enabled HNL-catalyzed preparations of optically active cyanohydrins on a technical scale. The enantioselectivity of chiral metal-complex-catalyzed additions of trimethylsilyl cyanide to aldehydes has been improved, but is, by far, not yet competitive with the HNL-catalyzed reactions.
由于醇腈酶（HNLs）具有良好的可及性、相对较高的稳定性以及易于操作的特点，（R）-和（S）-氰醇现在很容易获得。反应条件（溶剂、温度以及使用定点诱变等）的优化使得在工业规模上通过HNL催化制备光学活性氰醇成为可能。手性金属络合物催化的三甲基硅氰化物与醛的对映选择性加成反应的对映选择性已经得到改善，但到目前为止，仍无法与HNL催化的反应相竞争。
