Phillips D M
J Exp Zool. 1975 Jan;191(1):1-8. doi: 10.1002/jez.1401910102.
Replicas of critical point dried rodent sperm were examined by transmission electron microscopy. The surface of rat sperm heads appeared to be coated with regularly spaced 90A lamellar material. The plasma membrane overlying the acrosomal region of guinea pig spermatozoa displayed a regular scalloped array of lamellar structures. In replicas, the surface of Chinese hamster spermatozoa appeared coated by an array of small tubles and vesicles in the region overlaying the acrosome. It was possible to obtain replicas of the outer acrosomal membrane by removing the plasma membrane of mouse spermatozoa by brief treatment with Hank's balanced salt solution containing 0.2-0.5% MgC12. Replicas of the surface of the outer acrosomal membrane reveal evenly spaced, hexagonally-packed 90A particles similar to those which have been observed by other workers in replicas of freeze fractured outer acrosomal membranes. The finding of hexagonally arranged structures on the surface of the outer acrosomal membrane which appear very similar to those which have been observed in the plane of the membrane suggests that protein molecules in the plane of the membrane may protrude through or in some other manner distort the membrane surface. The post-acrosomal region of mouse sperm displays parallel lamellae with 100A spacing. If surface changes occur on sperm heads during maturation or capacitation, it should be possible to detect them with this technique.
通过透射电子显微镜检查了临界点干燥的啮齿动物精子的复制品。大鼠精子头部表面似乎覆盖着规则间隔的90埃层状物质。豚鼠精子顶体区域上方的质膜呈现出规则的扇贝状层状结构排列。在复制品中，中国仓鼠精子顶体上方区域的表面似乎被一系列小的微管和小泡所覆盖。通过用含有0.2 - 0.5% MgC12的汉克平衡盐溶液短暂处理去除小鼠精子的质膜，可以获得顶体外膜的复制品。顶体外膜表面的复制品显示出均匀间隔、六边形排列的90埃颗粒，类似于其他研究人员在冷冻断裂的顶体外膜复制品中观察到的颗粒。在顶体外膜表面发现六边形排列的结构，它们与在膜平面上观察到的结构非常相似，这表明膜平面中的蛋白质分子可能会穿过膜表面或以其他方式使膜表面变形。小鼠精子的顶体后区域显示出间距为100埃的平行薄片。如果精子头部在成熟或获能过程中发生表面变化，应该可以用这种技术检测到。
