Hoffman L H, Wimsatt W A, Olson G E
Am J Anat. 1987 Apr;178(4):326-34. doi: 10.1002/aja.1001780404.
Plasma membrane structure of bat spermatozoa was examined utilizing electron microscopy of thin sections and freeze-fracture replicas. Notable membrane features observed in replicas from cauda epididymal spermatozoa included specialized particle aggregates at the junction between the acrosomal and postacrosomal region of the head (a membrane structure not previously described in mammalian spermatozoa) and another row of rod-like particles just anterior to the posterior ring. Both of these specializations in fractured plasma membranes correspond with regions where the membrane is closely apposed to underlying structures when viewed in thin sections. The postacrosomal sheath appears to be composed of an array of longitudinally oriented filamentous components. Characteristic ordering of intramembranous particles was also noted in replicas from the midpiece region and the annulus. Major changes in plasma membrane structure were not seen in spermatozoa stored in the female reproductive tract; however, the appearance of linear particle aggregations in the principal piece membrane was noted. No evidence was obtained to suggest that an acrosome reaction had occurred in spermatozoa stored in females.
利用薄切片电子显微镜和冷冻断裂复型技术对蝙蝠精子的质膜结构进行了研究。在附睾尾部精子的复型中观察到的显著膜特征包括头部顶体和顶体后区域交界处的特殊颗粒聚集体（一种此前未在哺乳动物精子中描述过的膜结构）以及后环前方的另一排棒状颗粒。在薄切片中观察时，断裂质膜中的这两种特殊结构都与膜紧密贴附于下方结构的区域相对应。顶体后鞘似乎由一系列纵向排列的丝状成分组成。在中段区域和环的复型中也注意到了膜内颗粒的特征性排列。储存在雌性生殖道中的精子质膜结构未见重大变化；然而，在主段膜中注意到了线性颗粒聚集体的出现。没有证据表明储存在雌性体内的精子发生了顶体反应。
