Lowe T P, Larkin J R
J Exp Zool. 1975 Jan;191(1):25-32. doi: 10.1002/jez.1401910104.
To gain insight into the sex-determining mechanism of the Siamese fighting fish, Betta splendens, sex-reversed individuals were bred and the ratios of the spawnings were examined. Sex-reversal of 245 females was undertaken by ovariectomizing them; of these, 104 became sex-reversed. Twenty-three of these latter fish were mated to normal females and eleven spawnings were raised to maturity. These spawnings resulted in all female broods or mixed broods. Were the male fish heterogametic, a view currently held by some authors, no males would be produced in these spawnings. Thus, male heterogamety was not substaintiated in this study. Contrary to other studies, the experimental sex reversal of females is not a rare event since nearly two-thirds of the fish that survived the surgery became sex-reversed. Gross dissection and histological observation of sex-reversed fish revealed a regenerated, unpaired duct which remained after the ovaries had been removed. The tissue of the regenerate was testicular and contained active spermatogenesis. Some alterative methods of sex determination which may apply to the Betta are examined. These include the possibility of two different sex-determining races, the effects of exogenous factors, and a polygenic system of sex determination.
为深入了解暹罗斗鱼（Betta splendens）的性别决定机制，对性逆转个体进行了繁殖，并检查了产卵比例。对245只雌性斗鱼进行卵巢切除以实现性逆转；其中，104只实现了性逆转。将其中23只性逆转后的鱼与正常雌性斗鱼交配，并将11窝鱼苗饲养至成熟。这些鱼苗全部发育为雌性或为雌雄混合。如果雄鱼是异配性别（目前一些作者持这种观点），那么在这些鱼苗中就不会产生雄鱼。因此，本研究并未证实雄鱼异配性别这一观点。与其他研究相反，雌性斗鱼的实验性性逆转并非罕见事件，因为在手术后存活下来的鱼中，近三分之二实现了性逆转。对性逆转后的斗鱼进行大体解剖和组织学观察发现，卵巢切除后留下了一条再生的、不成对的管道。该再生组织为睾丸组织，含有活跃的精子发生过程。研究了一些可能适用于斗鱼的其他性别决定方法。这些方法包括存在两种不同性别决定类型的可能性、外源因素的影响以及多基因性别决定系统。
