Li Y, Tian S, Douglas S D, Ho W Z
Division of Immunologic and Infectious Diseases, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Cell Immunol. 2000 Nov 1;205(2):120-7. doi: 10.1006/cimm.2000.1713.
In vitro and in vivo studies have indicated that there is an important relationship between morphine and neuropeptide substance P (SP). We therefore investigated the interaction of morphine and cultured human immune cells on the expression of SP, a neuropeptide which we have recently demonstrated to be produced by human monocytes and lymphocytes. Morphine up-regulated SP production in human mononuclear phagocytes and lymphocytes at both the mRNA and the protein level. In addition, morphine induced SP receptor (NK-1R) expression in human lymphocytes. The specific morphine receptor antagonist (naltrexone) blocked morphine-induced SP expression in human mononuclear phagocytes, supporting the concept of authentic morphine receptor-mediated regulation. Since SP modulates neurogenic inflammation and immunologic events, these data suggest that morphine-induced SP expression in cells of the immune system may be of importance in the pathogenesis of immune-mediated diseases, including neuroimmunologic diseases and AIDS.
体外和体内研究表明，吗啡与神经肽P物质（SP）之间存在重要关系。因此，我们研究了吗啡与培养的人免疫细胞对SP表达的相互作用，SP是一种我们最近证明由人单核细胞和淋巴细胞产生的神经肽。吗啡在mRNA和蛋白质水平上均上调人单核吞噬细胞和淋巴细胞中SP的产生。此外，吗啡诱导人淋巴细胞中SP受体（NK-1R）的表达。特异性吗啡受体拮抗剂（纳曲酮）可阻断吗啡诱导的人单核吞噬细胞中SP的表达，支持了真正的吗啡受体介导调节的概念。由于SP调节神经源性炎症和免疫事件，这些数据表明，吗啡诱导免疫系统细胞中SP的表达可能在免疫介导疾病（包括神经免疫疾病和艾滋病）的发病机制中具有重要意义。
