Goode T, O'Connell J, Sternini C, Anton P, Wong H, O'Sullivan G C, Collins J K, Shanahan F
Department of Medicine, National University of Ireland, Cork.
J Immunol. 1998 Sep 1;161(5):2232-40.
Reciprocal communication between the immune system and the neuroendocrine system is mediated via a common chemical language of shared ligands and receptors. The neuropeptide substance P (SP) has been implicated as a mediator of immunomodulation. The evidence for substance P receptors on human lymphocytes is, however, controversial. The aims of the present study are to investigate substance P receptor (SPR) expression in human peripheral and mucosal mononuclear cells and to identify cellular sites of expression in human colonic mucosa. Using reverse-transcriptase PCR, we demonstrate that PBMC isolations are negative for SPR mRNA expression, whereas lamina propria mononuclear cell (LPMC) isolations express on average eight SPR mRNA transcripts per cell. In situ hybridization performed on surgically resected colonic tissue confirms the expression of SPR mRNA in LPMC in vivo. SPR mRNA signal was detected in LPMC, lymphoid follicles, and epithelium. The complementary technique of immunohistochemistry gave a similar distribution of SPR expression that colocalized with CD45 immunoreactivity. Dual-fluorochrome flow cytometry revealed SPR expression by CD4, CD45RO, CD45RA, CD8, CD19, and CD14 LPMC subsets, but not PBMC. Our findings suggest that SPR expression is distinctive of human colonic mucosal mononuclear cells and support a direct role for SP in mucosal immunomodulation.
免疫系统与神经内分泌系统之间的相互交流是通过共享配体和受体这一共同化学语言介导的。神经肽P物质（SP）被认为是免疫调节的介质。然而，关于人类淋巴细胞上P物质受体的证据存在争议。本研究的目的是调查人类外周和黏膜单核细胞中P物质受体（SPR）的表达，并确定人类结肠黏膜中的表达细胞位点。使用逆转录聚合酶链反应，我们证明外周血单个核细胞（PBMC）分离物中SPR mRNA表达呈阴性，而固有层单核细胞（LPMC）分离物平均每个细胞表达8个SPR mRNA转录本。对手术切除的结肠组织进行原位杂交证实了LPMC在体内SPR mRNA的表达。在LPMC、淋巴滤泡和上皮细胞中检测到SPR mRNA信号。免疫组织化学的互补技术给出了与CD45免疫反应性共定位的类似SPR表达分布。双荧光流式细胞术显示CD4、CD45RO、CD45RA、CD8、CD19和CD14 LPMC亚群表达SPR，但PBMC不表达。我们的研究结果表明，SPR表达是人类结肠黏膜单核细胞所特有的，并支持SP在黏膜免疫调节中发挥直接作用。
