Ludkiewicz B, Wójcik S, Spodnik E, Domaradzka-Pytel B, Moryś J
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Poland.
Folia Morphol (Warsz). 2000;59(4):263-70.
Immunohistochemical study of the cholinergic innervation of the hippocampal cells containing glutamic acid decarboxylase (GAD) and calcium binding proteins: parvalbumin (PV), calbindin D28k (CB) and calretinin (CR) was conducted on 5 adult rat brains. Analysis of sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and respectively either GAD or PV, CB, CR, using confocal laser-scanning microscope shows that the intensive cholinergic innervations receive GAD, PV and CB-positive hippocampal cells. Cholinergic afferentiations of the CR-positive neurones are considerably fewer.
对5只成年大鼠脑进行了免疫组织化学研究，观察含有谷氨酸脱羧酶（GAD）和钙结合蛋白：小白蛋白（PV）、钙结合蛋白D28k（CB）和钙视网膜蛋白（CR）的海马细胞的胆碱能神经支配。使用共聚焦激光扫描显微镜对囊泡乙酰胆碱转运体（VAChT；胆碱能细胞、纤维和终末的标志物）与分别为GAD或PV、CB、CR进行双重免疫染色的切片分析表明，密集的胆碱能神经支配接受GAD、PV和CB阳性的海马细胞。CR阳性神经元的胆碱能传入明显较少。
