Geloso M C, Vinesi P, Michetti F
Institute of Histology, Catholic University, Rome, 00168, Italy.
Exp Neurol. 1998 Dec;154(2):645-53. doi: 10.1006/exnr.1998.6949.
The present study investigates, by immunocytochemistry, the behavior of different neuronal subpopulations of the developing rat hippocampus, selectively labeled by the calcium-binding proteins calbindin D28-k (CB), parvalbumin (PV), and calretinin (CR), in neurodegenerative processes induced by the neurotoxicant trimethyltin (TMT). Previous studies on adult rats indicated that CB-immunoreactive (IR) neurons were affected by TMT, while PV- and CR-IR neurons were selectively spared. The present findings show that only CR-IR neurons are spared in developing rats, and in addition the number of CR-IR neurons are significantly higher in the DG of treated animals. On the contrary, PV-IR neurons, spared in adult rats, were affected by TMT during development. CB-IR neurons were affected also in developing rats, as in adults. The different postnatal time-courses of calcium-binding protein expression in relationship to the time of TMT administration (presence of CR but absence of PV) could have a role in the different behavior of CR- and PV-IR cells in developing rats.
本研究通过免疫细胞化学方法，研究了发育中大鼠海马体不同神经元亚群的行为，这些亚群由钙结合蛋白钙结合蛋白D28-k（CB）、小白蛋白（PV）和钙视网膜蛋白（CR）选择性标记，在神经毒物三甲基锡（TMT）诱导的神经退行性过程中的表现。先前对成年大鼠的研究表明，CB免疫反应性（IR）神经元受TMT影响，而PV-和CR-IR神经元则选择性地幸免。目前的研究结果表明，在发育中的大鼠中只有CR-IR神经元幸免，此外，在接受治疗的动物的齿状回中，CR-IR神经元的数量显著增加。相反，在成年大鼠中幸免的PV-IR神经元在发育过程中受到TMT的影响。CB-IR神经元在发育中的大鼠中也像在成年大鼠中一样受到影响。钙结合蛋白表达与TMT给药时间（存在CR但不存在PV）的不同出生后时间进程，可能在发育中大鼠的CR-和PV-IR细胞的不同行为中起作用。
