Tollersrud T, Kenny K, Caugant D A, Lund A
National Veterinary Institute, Oslo, Norway.
APMIS. 2000 Sep;108(9):565-72. doi: 10.1034/j.1600-0463.2000.d01-98.x.
Eighty-six Staphylococcus aureus isolates from cases of bovine mastitis were characterised biochemically and with respect to serotype, multilocus enzyme electrophoresis genotypes, antibiotic sensitivity, and production of enterotoxins A through D (SEA-D) and toxic shock syndrome toxin-1 (TSST-1). The samples were obtained from 81 different cows from 79 Norwegian dairy herds in 10 different counties in southern Norway. There was an equal representation of isolates from cases of acute, chronic and subclinical mastitis. Multilocus enzyme electrophoresis using 13 genetic loci showed that 69 of 86 isolates had the same electrophoretic type. This common electrophoretic type comprised isolates that differed in the expression of other phenotypical characteristics studied. Fifty-eight percent of the isolates produced one or more enterotoxins, predominantly a combination of SEC and TSST-1. Capsular serotyping revealed that 95% of the isolates belonged to serotype 8. No correlation was found between the factors studied and the clinical classification of mastitis. It appears that the majority of S. aureus isolates recovered from cases of bovine mastitis in Norway are genetically closely related and express common phenotypical characteristics.
对86株从牛乳腺炎病例中分离出的金黄色葡萄球菌进行了生化特征分析，并分析了其血清型、多位点酶电泳基因型、抗生素敏感性以及A至D型肠毒素（SEA-D）和毒性休克综合征毒素-1（TSST-1）的产生情况。样本取自挪威南部10个不同郡的79个挪威奶牛场的81头不同奶牛。急性、慢性和亚临床乳腺炎病例的分离株数量相当。利用13个基因位点进行的多位点酶电泳显示，86株分离株中有69株具有相同的电泳类型。这种常见的电泳类型包含的分离株在其他所研究的表型特征表达上存在差异。58%的分离株产生一种或多种肠毒素，主要是SEC和TSST-1的组合。荚膜血清分型显示，95%的分离株属于8型血清型。在所研究的因素与乳腺炎的临床分类之间未发现相关性。看来从挪威牛乳腺炎病例中分离出的大多数金黄色葡萄球菌分离株在基因上密切相关，并表现出共同的表型特征。
