Holm-Hansen C, Stern B, Rustad S, Shao J, Asjö B
Centre for International Health, University of Bergen, Norway.
APMIS. 2000 Sep;108(9):608-16. doi: 10.1034/j.1600-0463.2000.d01-104.x.
The aim of this study was to determine HIV-1 V3 sequences, in vitro biological characteristics and co-receptor usage of virus isolates from Tanzania. Virus was isolated from 14 of 17 samples investigated. Four of the isolates induced syncytia in MT-2 cells and used the CXCR4 co-receptor, while the remaining 10 isolates used the CCR5 co-receptor characteristic of non-MT-2 tropic viruses. One of the four MT-2 tropic isolates also used the CCR5 and CCR3 co-receptors. Proviral DNA was detected in all 14 isolates and PCR products were subjected to DNA sequencing. Unambiguous V3 amino acid sequences were obtained from 11 amplificates. Phylogenetic analysis indicated that these sequences were divergent and clustered in HIV-1 subtypes A, C or D. Sequences from the viruses that induced syncytia in MT-2 cells presented characteristic V3 phenotype-associated amino acids. Results of co-receptor analysis are in concordance with the isolate phenotype as determined by replication and induction of syncytia in MT-2 cells. The considerable diversity illustrated by a limited number of isolates from Tanzania is in accordance with reports from other regions of Africa.
本研究的目的是确定来自坦桑尼亚的病毒分离株的HIV-1 V3序列、体外生物学特性和共受体使用情况。在17个被调查的样本中，从14个样本中分离出了病毒。其中4个分离株在MT-2细胞中诱导形成合胞体，并使用CXCR4共受体，而其余10个分离株使用非MT-2嗜性病毒特有的CCR5共受体。4个MT-2嗜性分离株中的1个还使用CCR5和CCR3共受体。在所有14个分离株中均检测到前病毒DNA，并对PCR产物进行DNA测序。从11个扩增产物中获得了明确的V3氨基酸序列。系统发育分析表明，这些序列存在差异，并聚类于HIV-1 A、C或D亚型中。在MT-2细胞中诱导形成合胞体的病毒序列呈现出与V3表型相关的特征性氨基酸。共受体分析结果与通过在MT-2细胞中的复制和合胞体诱导所确定的分离株表型一致。坦桑尼亚数量有限的分离株所显示出的显著多样性与非洲其他地区的报告一致。
