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诱导三倍体对虹鳟幼鱼(Oncorhynchus mykiss)鳍再生的影响。

Effect of induced triploidy on fin regeneration of juvenile rainbow trout, Oncorhynchus mykiss.

作者信息

Alonso M, Tabata Y A, Rigolino M G, Tsukamoto R Y

机构信息

Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Brazil.

出版信息

J Exp Zool. 2000 Dec 1;287(7):493-502. doi: 10.1002/1097-010x(20001201)287:7<493::aid-jez5>3.0.co;2-8.

DOI:10.1002/1097-010x(20001201)287:7<493::aid-jez5>3.0.co;2-8
PMID:11110163
Abstract

Triploidy was induced in the rainbow trout in order to evaluate whether the altered numbers and sizes of triploid cells could modify fin regeneration. Amputation of one lobe of the tail fin of diploid and triploid juveniles resulted in regeneration for experimentals and controls. Nevertheless, both rate and frequency of regeneration in triploids were significantly increased as compared with diploids, a fact that can be attributed to the increased nuclear and cellular volume in a wide range of tissues, whereas the cell numbers were reduced. These data suggest that a great deal of interesting and important research could be done using triploid animals as experimental models for studying the regeneration of appendages.

摘要

为了评估三倍体细胞数量和大小的改变是否会影响鳍的再生,人们诱导虹鳟鱼产生了三倍体。对二倍体和三倍体幼鱼的尾鳍一叶进行截肢后,实验组和对照组的鳍均发生了再生。然而,与二倍体相比,三倍体的再生速率和频率均显著提高,这一现象可归因于多种组织中细胞核和细胞体积的增大,而细胞数量减少。这些数据表明,使用三倍体动物作为研究附肢再生的实验模型,可以开展大量有趣且重要的研究。

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