成纤维细胞培养中的酸性粘多糖。1. 细胞密度、pH值和乳酸浓度对粘多糖分布模式的影响。

Acid mucopolysaccharides in fibroblast cultures. 1. Influence of cell density, pH-value and lactate concentration on the MPS distribution pattern.

作者信息

Kittlick P D, Neupert G, Bolck F

出版信息

Exp Pathol (Jena). 1976;12(3-4):149-58.

PMID:11111

Abstract

From cells and culture media of embryonic rat fibroblasts (1st subculture) the acid mucopolysaccharides were isolated and fractionated. The per cent calculation of the 6 fractions was based on the content of glucuronic acid. The cultures were maintained as follows: 0.5 X 10(6) to 3 X 10(6) cells were examined in Demeter flasks at pH 7.4 or 6.6; lactate concentration was enhanced to 100 mg%. The amount of each fraction was correlated with the cell density (linear regression). The pH-value and lactate concentration in connection with cell density proved to be important factors in the modification of the MPS distribution pattern.

摘要

从胚胎大鼠成纤维细胞（第1代传代培养）的细胞和培养基中分离并分级分离酸性粘多糖。6个级分的百分比计算基于葡萄糖醛酸的含量。培养条件如下：在pH 7.4或6.6的德梅特烧瓶中检测0.5×10⁶至3×10⁶个细胞；乳酸浓度提高到100mg%。将每个级分的量与细胞密度相关联（线性回归）。结果证明，pH值、乳酸浓度与细胞密度是改变粘多糖分布模式的重要因素。

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The glycosaminoglycans in trypsin-damaged fibroblast cultures.

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