成纤维细胞培养中的酸性粘多糖。2. 依赖于pH值和细胞密度的35S-硫酸盐掺入动力学。
Acid mucopolysaccharides in fibroblast cultures. 2. 35S-sulfate incorporation kinetics in dependence on pH-value and cell density.
作者信息
Kittlick P D, Neupert G, Stiller K J, Bolck F
出版信息
Exp Pathol (Jena). 1976;12(3-4):159-62.
PMID:11112
Abstract
Cultures of embryonic rat fibroblasts were incubated with 35S-sulfate at pH 6.6 and 7.4 (Eagle basal medium plus HEPES buffer) for 12 to 48 hours. The acid mucopolysaccharides were isolated and fractionated after the method of SVEJCAR and ROBERTSON. Sulfate incorporation was determined by liquid scintillation counting.
摘要
将胚胎大鼠成纤维细胞培养物在pH 6.6和7.4（伊格尔基础培养基加HEPES缓冲液）条件下与35S-硫酸盐一起孵育12至48小时。按照SVEJCAR和ROBERTSON的方法分离并分级分离酸性粘多糖。通过液体闪烁计数法测定硫酸盐掺入量。
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