Tseng L, Mazella J, Goligorsky M S, Rialas C M, Stefano G B
Department of Obstetrics/Gynecology, SUNY-Stony Brook, Stony Brook, New York, USA.
J Soc Gynecol Investig. 2000 Nov-Dec;7(6):343-7.
Previous studies have shown that human endometrial glandular epithelial cells contain endothelial nitric oxide synthase indicating that the endometrium might produce nitric oxide (NO). We conducted this study to identify stimuli that can activate a transient NO release from endometrial glandular epithelial cells because NO is an important intracellular and intercellular signal transduction pathway in reproductive cycle.
Endometrial glandular epithelial cells, free of endothelial cells, were isolated from human endometrial specimens and maintained viable in RPMI 1640 medium with 2% fetal bovine serum for 2-4 days. Nitric oxide release from the glandular cells in response to stimuli was monitored continuously amperometrically.
Among the substances examined, we found that dopamine and morphine stimulated a transient surge of NO production that was dose-dependent, whereas estrogen, progesterone, or relaxin (RLX) had no short-term effect on NO release. Cells treated with RLX or dopamine for 4 days enhanced the dopamine-induced NO release fourfold to sixfold, with the peak of the NO surge shifting from 35 to 15 seconds.
Endometrial glandular cells were capable of producing NO. Dopamine and morphine were potent stimuli for a transient surge of NO release from endometrial glandular cells. Furthermore, prolonged exposure to dopamine or RLX enhanced the sensitivity of NO release in endometrial glands.
以往研究表明，人子宫内膜腺上皮细胞含有内皮型一氧化氮合酶，提示子宫内膜可能产生一氧化氮（NO）。我们开展本研究以确定能够激活子宫内膜腺上皮细胞短暂释放NO的刺激因素，因为NO是生殖周期中一种重要的细胞内和细胞间信号转导途径。
从人子宫内膜标本中分离出不含内皮细胞的子宫内膜腺上皮细胞，并在含2%胎牛血清的RPMI 1640培养基中维持存活2 - 4天。通过安培法连续监测腺细胞对刺激作出反应时的一氧化氮释放情况。
在所检测的物质中，我们发现多巴胺和吗啡可刺激NO产生短暂激增，且呈剂量依赖性，而雌激素、孕酮或松弛素（RLX）对NO释放无短期影响。用RLX或多巴胺处理细胞4天可使多巴胺诱导的NO释放增加4至6倍，NO激增峰值从35秒移至15秒。
子宫内膜腺细胞能够产生NO。多巴胺和吗啡是促使子宫内膜腺细胞短暂释放NO激增的有效刺激因素。此外，长期暴露于多巴胺或RLX可增强子宫内膜腺中NO释放的敏感性。
